Protein Info for CCNA_02640 in Caulobacter crescentus NA1000

Annotation: phospho-N-acetylmuramoyl-pentapeptide- transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 transmembrane" amino acids 26 to 48 (23 residues), see Phobius details amino acids 77 to 95 (19 residues), see Phobius details amino acids 101 to 119 (19 residues), see Phobius details amino acids 139 to 158 (20 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 209 to 229 (21 residues), see Phobius details amino acids 249 to 266 (18 residues), see Phobius details amino acids 273 to 292 (20 residues), see Phobius details amino acids 298 to 321 (24 residues), see Phobius details amino acids 348 to 367 (20 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 41 to 370 (330 residues), 385.5 bits, see alignment E=1.2e-119 PF00953: Glycos_transf_4" amino acids 104 to 293 (190 residues), 111.9 bits, see alignment E=1.6e-36

Best Hits

Swiss-Prot: 100% identical to MRAY_CAUVN: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 100% identity to ccs:CCNA_02640)

MetaCyc: 51% identical to phospho-N-acetylmuramoyl-pentapeptide-transferase (Escherichia coli K-12 substr. MG1655)
Phospho-N-acetylmuramoyl-pentapeptide-transferase. [EC: 2.7.8.13]

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8H097 at UniProt or InterPro

Protein Sequence (370 amino acids)

>CCNA_02640 phospho-N-acetylmuramoyl-pentapeptide- transferase (Caulobacter crescentus NA1000)
MLYLLYEWLARSQEHLPALNLLKYLTFRSGMAMLTAYIVAVAMGSRFIRWMKAKQGKGQP
IRTDGIARHVTEKAGTPTMGGFMILAGLFVAALLWADLRNVHVWVVLLITGSYGLLGFMD
DYAKVTKQTTAGLSSVQKLVAQFIVAIIATVILILFAPKSPMTPGMETSLVFPIFKALVI
NLGWFYVAFAAFTIAGFSNAVNLTDGLDGLAIVPVMFAASTFGLIAYLVGNYKFADYLNL
HFAPGVGELAVLCGAIIGGGMGFLWYNAPPAKIFMGDTGSLALGGALGAIAVCAKHELVL
GIVGGLFVAEALSVMIQVAYFKKTGKRVFLMAPIHHHFEKLGWPESTVVIRFWIVSMILA
FIGLATLKLR