Protein Info for CCNA_02535 in Caulobacter crescentus NA1000

Annotation: DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 899 PF01751: Toprim" amino acids 3 to 115 (113 residues), 71.4 bits, see alignment E=1.2e-23 TIGR01051: DNA topoisomerase I" amino acids 4 to 631 (628 residues), 778.4 bits, see alignment E=3.9e-238 PF01131: Topoisom_bac" amino acids 130 to 552 (423 residues), 413.8 bits, see alignment E=1.8e-127 PF01396: zf-C4_Topoisom" amino acids 598 to 632 (35 residues), 56.3 bits, see alignment (E = 4.3e-19) PF13368: Toprim_C_rpt" amino acids 651 to 709 (59 residues), 35 bits, see alignment 2.2e-12 amino acids 713 to 764 (52 residues), 60.3 bits, see alignment 2.8e-20 amino acids 782 to 834 (53 residues), 84 bits, see alignment 1.2e-27

Best Hits

Swiss-Prot: 51% identical to TOP1_RICCN: DNA topoisomerase 1 (topA) from Rickettsia conorii (strain ATCC VR-613 / Malish 7)

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 97% identity to ccs:CCNA_02535)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3CA12 at UniProt or InterPro

Protein Sequence (899 amino acids)

>CCNA_02535 DNA topoisomerase I (Caulobacter crescentus NA1000)
MNVVVVESPAKAKTINKYLGSDYTVLASYGHIRDLPSKDGSVEPDNDFAMSWEVDAKASK
RVSDIVDAMKKADRLILATDPDREGEAISWHVLEVLNKKKALKDKTVQRVTFNAITKTAV
TEAMKAPRDLDMELVEAYLARRALDYLVGFTLSPVLWRKLPGSRSAGRVQSVALRLVVDR
ELEIERFKTQEYWTVDADVSAGSDPFLARLVKHEGKKLTKFDLGNETSALTAKAAVEAAV
FKVEAVEKKPGKRSPAPPFTTSTLQQEAARKLGFSAQRTMQAAQKLYEGVDIGGETVGLI
TYMRTDGVSMAPEAIFEARDVIGAVYGKEYVPDSPRMYKSKAKNAQEAHEAIRPTAMGRN
PGSLRLEPELGRLYELIWKRTIASQMESARIERTTVDLTSADGQTGLRATGQVVQFPGYL
AVYEEGRDDEGDEDSARLPAITEGASAKVLKSRADQHFTEPPPRYSEASLVKKMEELGIG
RPSTYASVLGVLRDREYVRMEKQRFIPEDKGRLVTAFLEQFFKRYVEYDFTAALEEKLDL
VSDGKLDWKEFLREFWKDFHAAVGEIAELRTTNVLDALNEALGPHIFPDKGDGSNPRLCP
TCGSGQLSLKTGKFGAFIGCSNYPECRYTRQLGVSEGDGDAESADKQLGLNPATGLAVWL
KNGRFGPYVEELAAEGSSDKPKRSSLPKGWIASAMDMEKALRLLSLPREVGKHPDDGKVI
TAGLGRFGPFVLHDGTYANLENADEVFDVGLNRAVAILADKRAGGGRPQRGAAAALAELG
NHPEDGKPIRVLSGRFGPYIKHGDTNANVPKGKDPAAITLEEALALIAERAAKGGAKKPA
KKAAATKSKAKAESDAPAKKTAAKKPAAKKPAAKKAAPKAKAKATAASDDGAAPWDDEG