Protein Info for CCNA_02389 in Caulobacter crescentus NA1000

Annotation: glucosamine-1-phosphate acetyltransferase/UDP-N-acetylglucosamine pyrophosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 13 to 446 (434 residues), 466.3 bits, E=5.2e-144
• PF00483: NTP_transferase
  • amino acids 14 to 166 (153 residues), 29.8 bits, E=1.1e-10
• PF01128: IspD
  • amino acids 14 to 130 (117 residues), 38.8 bits, E=2.2e-13
• PF12804: NTP_transf_3
  • amino acids 14 to 152 (139 residues), 74.3 bits, E=3.2e-24
• PF00132: Hexapep
  • amino acids 310 to 344 (35 residues), 35.9 bits, 1e-12
  • amino acids 388 to 422 (35 residues), 29.6 bits, 9.9e-11
• PF14602: Hexapep_2
  • amino acids 388 to 422 (35 residues), 26.9 bits, 7.9e-10

Best Hits

Swiss-Prot: 100% identical to GLMU_CAUVN: Bifunctional protein GlmU (glmU) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to ccr:CC_2304)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See B8GYT1 at UniProt or InterPro

Protein Sequence (462 amino acids)

>CCNA_02389 glucosamine-1-phosphate acetyltransferase/UDP-N-acetylglucosamine pyrophosphorylase (Caulobacter crescentus NA1000)
MTESVSKPVRPRAAVILAAGQGTRMKSPTPKVLHRLAGRTLLDHAIDAAEGLGCERIIVV
VGAHSPQVGESARKRLGPDATVIQDPPLGTGHAVLAAKDALADFHGDVVVTYADCPLTTA
PVIAPLFDLITHVAHVAVLGFEAQNPTGYGRLILAPGHVLLRIVEEKEADLATKQVKHCN
SGVLAADRAVLFDLLANVRNDNAKGEYYLTDVVGLAHERHLSTRTAFAPEASVQGVNAQA
ELAAAEAVWQQNRRKALMVDGVTMPAPDTVHLAWDTQIAGGAVVEQFVVFGPGVSVASGA
VIKAFSHLEGAVVGEGALIGPYARLRPGAEIGPDAHIGNFVEVKKVKVGAGAKANHLSYL
GDGSVGEKANIGAGTIFCNYDGFEKFETHVGKGAFIGSNSALVAPVRVGDGAMTGSGSVI
TKDVEDGALALSRADQTSKAGWATKFRAIKQAQKDKKKDKKA