Protein Info for CCNA_02204 in Caulobacter crescentus NA1000

Annotation: ketol-acid reductoisomerase/2-dehydropantoate 2-reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 PF07991: IlvN" amino acids 14 to 178 (165 residues), 241.7 bits, see alignment E=4.6e-76 TIGR00465: ketol-acid reductoisomerase" amino acids 16 to 327 (312 residues), 422.3 bits, see alignment E=5.1e-131 PF01450: IlvC" amino acids 184 to 327 (144 residues), 222.1 bits, see alignment E=5e-70

Best Hits

Swiss-Prot: 100% identical to ILVC_CAUVC: Ketol-acid reductoisomerase (NADP(+)) (ilvC) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 100% identity to ccs:CCNA_02204)

MetaCyc: 68% identical to acetohydroxyacid isomeroreductase (Cupriavidus necator H16)
Ketol-acid reductoisomerase. [EC: 1.1.1.86]; 1.1.1.86 [EC: 1.1.1.86]

Predicted SEED Role

"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.86

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GXW2 at UniProt or InterPro

Protein Sequence (339 amino acids)

>CCNA_02204 ketol-acid reductoisomerase/2-dehydropantoate 2-reductase (Caulobacter crescentus NA1000)
MRVYYDRDADLARILDRKIAIVGYGSQGHAHALNLRDSGIKNVAVALRAGSPTAKKAEGE
GLKVMTVAEAAAWADLIMILAPDEHQAAIYKNEIALNIRDGAALLFAHGLNVHFGLIEPK
DTIDVLMVAPKGPGHTVRGEYQKGGGVPCLIAVHHNATGNALDLGLAYASAIGGGRSGII
ETNFREECETDLFGEQAVLCGGTVELVRAGFETLVEAGYAPEMAYFECLHELKLIVDLMY
EGGIANMNYSISNTAEYGEYVTGPRIITPETKAEMKRVLEDIQSGKFVRDFMLENAVGQP
SFKATRRRSAEHQIEEVGARLRGMMPWIAKNKLVDQAKN