Protein Info for CCNA_02029 in Caulobacter crescentus NA1000

Annotation: NADH-quinone oxidoreductase chain D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 TIGR01962: NADH dehydrogenase (quinone), D subunit" amino acids 30 to 416 (387 residues), 586.7 bits, see alignment E=9.5e-181 PF00346: Complex1_49kDa" amino acids 144 to 416 (273 residues), 406.9 bits, see alignment E=1.7e-126

Best Hits

Swiss-Prot: 100% identical to NUOD_CAUVC: NADH-quinone oxidoreductase subunit D (nuoD) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K00333, NADH dehydrogenase I subunit D [EC: 1.6.5.3] (inferred from 100% identity to ccs:CCNA_02029)

MetaCyc: 76% identical to MbhL (Pyrococcus furiosus)
Ferredoxin hydrogenase. [EC: 1.12.7.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.12.7.2 or 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C9C5 at UniProt or InterPro

Protein Sequence (416 amino acids)

>CCNA_02029 NADH-quinone oxidoreductase chain D (Caulobacter crescentus NA1000)
MTGTPSPVAATEPVRDEPVVPAIPETPVRKFNINFGPQHPAAHGVLRLVLELDGEIVERV
DPHIGLLHRGTEKLMEARTYLQNIPYFDRLDYVAPMNQEHAFCLAIEKLLGVEVPIRGQI
IRVLYSEIGRILNHLLNVTTQAMDVGALTPPLWGFEEREKLMVFYERACGARLHSNYFRP
GGVHQDLPPELVEDIDTWAKAFPKICDDIEGLITDNRIFKQRNVDIGVVSKEEAISWGFS
GVMVRGSGIAWDLRRSQPYENYNDFEFDIPLGKNGDCYDRYLCRMQEMRESTKIIRQACE
MLRKTHGPVLSEDNKVAPPRRAEMKRSMEALIHHFKLYTEGFKTPAGEVYACVEAPKGEF
GVFVVSDGTNKPYRCKIRAPGFPHLAAMDWMNRGHQLADVSAILGSLDIVFGEIDR