Protein Info for CCNA_01982 in Caulobacter crescentus NA1000

Updated annotation (from data): glutamyl-tRNA(Glx) synthetase (EC 6.1.1.24)
Rationale: A conserved essential gene. This activity is necessary to explain glutamine synthesis. Similar to both glutamyl-tRNA(Glu) and glutamyl-tRNA(Glx) synthetases (i.e. from E. coli or the organellar enzyme from Arabidopsis, which is probably transamidating, see PMID:3340166)
Original annotation: glutamyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to SYE_CAUVC: Glutamate--tRNA ligase (gltX) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 100% identity to ccs:CCNA_01982)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" (EC 6.1.1.17, EC 6.1.1.24)

MetaCyc Pathways

tRNA charging (19/21 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
superpathway of heme b biosynthesis from glutamate (8/10 steps found)
tetrapyrrole biosynthesis I (from glutamate) (5/6 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
adenosylcobalamin biosynthesis II (aerobic) (15/33 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (14/36 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C8P5 at UniProt or InterPro

Protein Sequence (470 amino acids)

>CCNA_01982 glutamyl-tRNA(Glx) synthetase (EC 6.1.1.24) (Caulobacter crescentus NA1000)
MSNPTPTGVVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKFLIRVEDTDRERSTEAAV
AAIFEGLDWLGLKSDDEVIFQHTRAPRHVEVVHELLAKGRAYRCWMSIEELEVAREKARA
EGRAIRSPWRDAPEGDLSAPHVIRFKGPLDGETLVNDLVKGPVTFKNIELDDLVLLRADG
APTYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQAMDWAVPAFAHIPLIHGPDGAK
LSKRHGAQAVGEFADLGYIPEGMRNYLARLGWGHGDDEVFTDEQAISWFDVADVVKAPAR
LDWAKLNHINAQHLRKADDARLTALALAAAETRGEPLPADAAERIARTVPEVKEGAKTIL
ELVDHCAFALKTRPLALEEKTQKQLTEETVERLKRLRDQLAAAPDFDAATLETVLKSFAE
SEGVGFGKFGPALRGVLTGGAQAPDLNKTMAALSRDEAIGRLDDALAPRA