Protein Info for CCNA_01706 in Caulobacter crescentus NA1000

Updated annotation (from data): lacA: subunit of periplasmic glucoside 3-dehydrogenase (EC 1.1.99.13)
Rationale: Specifically important in carbon source D-Raffinose pentahydrate; carbon source Beta-Lactose; carbon source. Previously shown to be required for and induced by growth on lactose and to be a component of lactose 3-dehydrogenase (PMCID:PMC2863468)
Original annotation: BetA-family choline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 PF01266: DAO" amino acids 15 to 102 (88 residues), 28.7 bits, see alignment E=2.5e-10 PF00732: GMC_oxred_N" amino acids 15 to 347 (333 residues), 47.9 bits, see alignment E=3.9e-16 PF13450: NAD_binding_8" amino acids 18 to 46 (29 residues), 23.2 bits, see alignment (E = 1.7e-08) PF05199: GMC_oxred_C" amino acids 443 to 562 (120 residues), 84.8 bits, see alignment E=2.2e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to ccr:CC_1634)

MetaCyc: 100% identical to lactose 3-dehydrogenase catalytic subunit (Caulobacter vibrioides)
GLUCOSIDE-3-DEHYDROGENASE-RXN [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]

Predicted SEED Role

"Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site" in subsystem Respiratory dehydrogenases 1

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.13

Use Curated BLAST to search for 1.1.2.11 or 1.1.99.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C822 at UniProt or InterPro

Protein Sequence (579 amino acids)

>CCNA_01706 lacA: subunit of periplasmic glucoside 3-dehydrogenase (EC 1.1.99.13) (Caulobacter crescentus NA1000)
MANLNGRARRKNTYDAIVVGSGITGGIAAKELTEKGLKVLVLERGPMVRHLEDYPTAMLD
PWQSKYPQGKLPEAELNAHYKVQRRTGYTMTEQTQHFFVRDDEHPYTEENRFDWIRGYHV
GGRSLTWGRQSYRHSPIDFEANAREGIAVDWPIRYEDLAPWYEHVERFIGVSGQAEGLPH
FPDGHYQPPMELNCVEKAFKARSEARFPERRVTIGRTAHLTDPTEEQLALGRTKCQYRNL
CIRGCPFGAYYSSNSGGLIAAERTGNLVIRPNSIVTELIYDERAGRASGVRILDAETRKD
EEFHADVIFLCASALNSAWIMMNSTSSRFPNGFGNASDQLGRNVMDHHLGAGATGQAPEF
ADMYFSGRRPNGIYVPRFRNLGDAASKRSDYLRGFGYQGGAGRATWERDRGQGGRGFGAA
RKAALSQPGPWTMGLSGFGEMLPYADNRVTLNRDVEDKFGLPTLTMNVTMRDNEMAMRRD
MQAAAAEMLEAAGFQNVRAHDNGFAPGLGIHEMGTARMGRDPKTSVLNAHNQVHECKNVY
VTDGAAMASASCVNPSLTYMALTARAADHAVRARKRGEL