Protein Info for CCNA_01704 in Caulobacter crescentus NA1000

Updated annotation (from data): lacB: cytochrome c2 component of periplasmic glucoside 3-dehydrogenase (EC 1.1.99.13)
Rationale: Specifically important in carbon source Beta-Lactose, and also important for carbon sources salicin and raffinose. Previously proposed to be a component of lactose 3-dehydrogenase (PMCID:PMC2863468)
Original annotation: cytochrome c

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 120 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF13442: Cytochrome_CBB3" amino acids 22 to 72 (51 residues), 24.5 bits, see alignment E=2.8e-09 PF00034: Cytochrom_C" amino acids 25 to 119 (95 residues), 45.7 bits, see alignment E=1.4e-15

Best Hits

Swiss-Prot: 39% identical to CYC_FRIAG: Cytochrome c (CYTC) from Fritillaria agrestis

KEGG orthology group: K08738, cytochrome c (inferred from 100% identity to ccs:CCNA_01704)

MetaCyc: 99% identical to c-type cytochrome protein (Caulobacter vibrioides)

Predicted SEED Role

"Cytochrome c2" in subsystem Soluble cytochromes and functionally related electron carriers

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.13

Use Curated BLAST to search for 1.1.99.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3CA31 at UniProt or InterPro

Protein Sequence (120 amino acids)

>CCNA_01704 lacB: cytochrome c2 component of periplasmic glucoside 3-dehydrogenase (EC 1.1.99.13) (Caulobacter crescentus NA1000)
MMKVRLTLAAVFAALASGAVAAPSGQQLFEQRCGMCHSLQQAPGKMGPPLAGVVGRKAAS
FPGYTYSAALKGSGITWTADKLDAYSKAPTKVVPGTKMLLGAPNDAERAAVIAYLASVKK