Protein Info for CCNA_01658 in Caulobacter crescentus NA1000

Annotation: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 1 to 359 (359 residues), 388.2 bits, see alignment E=1.5e-120 PF02547: Queuosine_synth" amino acids 3 to 360 (358 residues), 419.4 bits, see alignment E=4.8e-130

Best Hits

Swiss-Prot: 100% identical to QUEA_CAUVN: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 100% identity to ccr:CC_1587)

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GVF3 at UniProt or InterPro

Protein Sequence (366 amino acids)

>CCNA_01658 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (Caulobacter crescentus NA1000)
MRLSDFDFDLPEDRIALRPAEPRDSARFLVVRPGQAVADHIVSDLPDFLRPGDALVFNDT
RVIPARLSGLREGRTTGGADGTPVAVEATLHRRLAPDRWSAFMRPGKRLKVGDRVAFGGH
EGRVGDLGRLDAVIAEKHDGGEVVLAFDLSGPDLDVGIAQHGDMPLPPYIAAKRGEDERD
RADYQTVYAREDGSVAAPTAGLHFTPQLLERLKAKGVSLHFVTLHVGAGTFLPVKTDEVS
EHRMHAEYGQVTQEIADALNAARAAGGRIVCVGTTSLRLLESATGEDGIVRPFADETAIF
ITPGYRFRTADVLMTNFHLPKSTLFMLVSAFAGTEAMRAAYEHAIATGYRFYSYGDSSLL
FKDETC