Protein Info for CCNA_01651 in Caulobacter crescentus NA1000

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 919 TIGR01063: DNA gyrase, A subunit" amino acids 17 to 901 (885 residues), 1108 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 41 to 506 (466 residues), 520.8 bits, see alignment E=3e-160 PF03989: DNA_gyraseA_C" amino acids 538 to 583 (46 residues), 48.1 bits, see alignment 6.1e-17 amino acids 590 to 636 (47 residues), 19.6 bits, see alignment 5e-08 amino acids 648 to 692 (45 residues), 40.2 bits, see alignment 1.9e-14 amino acids 695 to 742 (48 residues), 41.5 bits, see alignment 7e-15 amino acids 804 to 850 (47 residues), 41.6 bits, see alignment 6.8e-15 amino acids 854 to 899 (46 residues), 44.2 bits, see alignment 1e-15

Best Hits

Swiss-Prot: 56% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 100% identity to ccr:CC_1580)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C8L4 at UniProt or InterPro

Protein Sequence (919 amino acids)

>CCNA_01651 DNA gyrase subunit A (Caulobacter crescentus NA1000)
MSDDHNTIPADGSRGDIAPINIEDELRRSYLDYAMSVIVSRALPDARDGLKPVHRRVLFS
MHEQGQTPERPYVKSARVVGDVMGKYHPHGDASIYFTLVRMTQSFSMGLVLIDGQGNFGS
VDGDMPAAMRYTECRMAPPAMALLADLDKDTVDFADNYDGKEQEPTVLPSRIPNLLVNGA
GGIAVGMATNIPPHNLGEVIDACLLLIDQPDVTTDQLLDLVPGPDFPTGGEIIGRAGPRQ
ALLTGRGSVIMRGVASVEELRAGREAIIVTEIPYQVNKANLVEHIAELVRDKKIEGVADI
RDESNRDGMRIVVELKRDASGEVILNQLYRFTALQSSFGVNMLALNRGRPEQMGLHQLIQ
LFVDFREEVVVRRTKFELGKARDRGHVLVGLTIAVANIDEFIHIIRSSKDPTEARERLVA
KSWPAGDMLPLVELIADPRTVQEDGGLIRLTDEQARAILALTLSRLTGLGRDEIGNEAAA
LADAIRGYLELLSDRANIMAVVREELVEVREKFAIPRRCQIVDGDADMEDEDLIVREDMV
ITVTMGGYVKRTPLANYRTQHRGGKGKSGMATKNEDAVTRVFSASTHAPLLFFTSGGKVY
KMKVWRLPLGVANSRGKAFVNLLPIEPGETITSILALPEDEATWGGLDVMFATRSGSVRR
NKLSDFVDVRRNGKIAMKLDEGDGIVGVAVCNADQDVLLTTAAGRCIRFSVDEVRVFASR
DSTGVRGVKLLDGDQVISMAVLRSVDATPAERAAYLKHQRAMLRAADGEEGEDTATAADD
GDEEVGEASLTPERIAELGAAEEILLTVSSEGFGKRTSAYDFRRTGRGGQGLAAQDLSKR
GGRLVGSFPIDESDQILLVTDQGQLIRVPVSQIRVAARNTQGVTIFRTAQDEHVVSVERL
ADSGGDDNQGEDSGADETP