Protein Info for CCNA_01622 in Caulobacter crescentus NA1000

Annotation: Rsh ppGpp hydrolase-synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 742 PF13328: HD_4" amino acids 45 to 194 (150 residues), 150.5 bits, see alignment E=1e-47 TIGR00691: RelA/SpoT family protein" amino acids 45 to 735 (691 residues), 649.3 bits, see alignment E=3.6e-199 PF01966: HD" amino acids 64 to 163 (100 residues), 40.4 bits, see alignment E=1e-13 PF04607: RelA_SpoT" amino acids 255 to 364 (110 residues), 138.3 bits, see alignment E=3.9e-44 PF02824: TGS" amino acids 414 to 472 (59 residues), 73.4 bits, see alignment 3.6e-24 PF19296: RelA_AH_RIS" amino acids 485 to 650 (166 residues), 109.7 bits, see alignment E=5.9e-35 PF13291: ACT_4" amino acids 667 to 739 (73 residues), 61.5 bits, see alignment E=3.2e-20

Best Hits

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 100% identity to ccr:CC_1553)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C9W6 at UniProt or InterPro

Protein Sequence (742 amino acids)

>CCNA_01622 Rsh ppGpp hydrolase-synthetase (Caulobacter crescentus NA1000)
MSSVAPVAVTEAPAKPRPKFLRQYELIERVHAYDPTADEALLNRAYVYAMRMHGSQTRAS
GDPYYAHPIEVAGILTEYRLDTATIVTALLHDVIEDTPVTKEEIAKLFGEEIGELVEGVT
KLSKLELQAEHMRQAENLRKFILAISKDVRVLLVKLADRLHNMRTLHFIKNQAKRERIAR
ETRDIYAPLARNIGCHRICTELEELSFQHTNPVARDAIIRRLDVLREEQGGAVTLVSQEI
AARLESASLPARVFGREKSPYSIWRKLQRKSIGFSQMSDIYAFRVIVDSEEDCYRALGVV
HRAWSSVPDRFKDYISTPKRNNYRSLHTTVVGPRGMRIEMQIRTESMDRVNEEGVAAHFR
YKDASYGLDLEGMEAAGGRDPLANLRQLVQVLEHGGDSEELVEHAKLEMFLDQVFVFTPK
GKLVSLPRGAMPLDFAYAVHTSVGDTCIGVKINGELKPLRTPLVNGDVVEVVRGSKPVVP
PDWRSLTVTGRARSAIRRHIRQTEKEEFLRLGRASLEQVFERAGKKLKDVSLRPILERFV
LETDEALFDAVGRGRVSPSQVLETAYPGMKDSEREAATARRKIEGGQEAARLYVRGGGLT
PGVSLHFAHCCSAVPGDRIVGILREDGEGLDVHTIDCPRLAEYEDREELWRDLNWTPEAE
RSTISLTRLHATIQNAPGVLGLVCTIIGEAGGNIVNLRMHHRQSDFFDTDIDVEVRDAKH
LTNIQAALRACPSVETVDRTRG