Protein Info for CCNA_01596 in Caulobacter crescentus NA1000

Annotation: DNA helicase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 PF00580: UvrD-helicase" amino acids 43 to 308 (266 residues), 299.1 bits, see alignment E=9.6e-93 PF13245: AAA_19" amino acids 47 to 291 (245 residues), 76.1 bits, see alignment E=7.9e-25 PF13361: UvrD_C" amino acids 313 to 642 (330 residues), 223.1 bits, see alignment E=1.9e-69

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 100% identity to ccr:CC_1528)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C7U0 at UniProt or InterPro

Protein Sequence (805 amino acids)

>CCNA_01596 DNA helicase II (Caulobacter crescentus NA1000)
MFPDTDSPSPIGDFPGDLPSPRISDLARARPPAGAAPGYLDGLNPEQREAVETTEGPLLV
LAGAGTGKTRVLTTRLAHILATGRARPWEILAVTFTNKAAREMRERITHIIGPEAEGLRW
LGTFHSVAAQILRRHAELIGLKSNYTIIDTDDNERLLKQLIEAENIDAKRWTPRILSQII
DGWKNRGWTPDRVPTSEAGEFANGKGISLYRQYQERLRILNACDFGDLLLHNISLFTGHP
DVLDEFQQRFKYVMVDEYQDTNVAQYLWLRLLAQGRQNVCCVGDDDQSIYGWRGAEVDNI
LRFERDFPGAKVVRLECNYRSTEHILAAASGLITANKGRLGKTLWTPSKGGEKVKVSGVW
DGEAESRLIADEIERAKKAGRKYSQMAILVRASFQMRAFEERFVLLQIPYKVVGGPRFFE
RAEIRDAHAYLRLIQSPDDDLAFERIVNTPKRGIGDTSVQKLLQIARLGGISTVEAARQI
ITSDELPARTRTALSNFIRDLDRWRSLVGTAHHQRLLETVLEESGYTDALRLDKGPTSQT
RLENLKELVQSMGAFDTLEAYLEHVSLVMDLDREATGDAVWIMTLHSAKGLEFPLVFLPG
WEEGVFPSQRSMDDKGEKGLEEERRLAYVGITRAREEARISFVANRQVYGRWTSQLPSRF
VDELPIAHVDAASETGYYGGGPGMQSTAASRWDDPGTSAFQAGSYDSPGWKRAQERTSGF
GERGGSWRGAQGVRSGSSARSAPIEGEGRLVAVSAPTNSAYARGDRVFHVKFGYGKVTGI
EGNKLTVAFDKAGEKKVIDSFVEKA