Protein Info for CCNA_01590 in Caulobacter crescentus NA1000

Annotation: NAD-dependent DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 783 PF01653: DNA_ligase_aden" amino acids 14 to 330 (317 residues), 372.5 bits, see alignment E=4.2e-115 TIGR00575: DNA ligase, NAD-dependent" amino acids 21 to 777 (757 residues), 718.6 bits, see alignment E=3.7e-220 PF03120: DNA_ligase_OB" amino acids 334 to 409 (76 residues), 112.5 bits, see alignment E=1.7e-36 PF00533: BRCT" amino acids 711 to 780 (70 residues), 52.8 bits, see alignment E=9.7e-18

Best Hits

Swiss-Prot: 100% identical to DNLJ_CAUVN: DNA ligase (ligA) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to ccr:CC_1522)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8H5Y7 at UniProt or InterPro

Protein Sequence (783 amino acids)

>CCNA_01590 NAD-dependent DNA ligase (Caulobacter crescentus NA1000)
MSQIPVADLTEAQAVEDLERLADLLATHDIAYHQEDNPTVSDAEYDALKRRNLDIETRFP
HLVRDNSPSMRVGATRAEQFAPVEHGVPMLSLDNAFSNDEAIEFDARVRRFLRISPSETV
AYTAEPKIDGLSASLRYEKGVLVQGATRGDGRVGEDVTANLRTIADIPHRLKGSGWPDVI
EVRGEVYVELAAFAAFNKAAEEAGQRTYANPRNFAAGSLRQIDPKISAQRPLRFFGYAWG
LVSEGFADSQWGALERLAEWGFVTTAPPAQRVLNAQGLLDIYAQFEVLRPTLGFDIDGVV
YKVDDLELQRRLGFVSRSPRWAIARKFPAQRARTVLEAIDLQVGRTGAITPVARLKPVTV
GGVSVTNATLHNGDEIARLDVRVGDTVVIQRAGDVIPQIVEVALDARPDPAPPPYEFPHV
CPCPLQTPLAREVTASGQESVVRRCTGEFACPFQRVEHLRHFVSRRAFDIEGLGEKQLQA
FFEEGWITEPADIFKLARDAEKLAVLREREGYGETSVANLVKGIEARRTIGMDRMIYGLG
ARDIGETTSTVLARNFDRFEDLQAAAEAAARALPGETYLELSTAPGVGPKALDMLVEAGK
GGVVADPWPQTDDLELKIGHAVPKLTKPARAALAQRYGTWDAFADGLVAAASGAPGDDYL
HLAAIDGVGPVAAQSLARFFAEDHNRQKVANLVAELDIQPVAKPKTDTAVAGKTIVFTGS
LEKMTRDEAKAQAEGLGAKVASSVSKKTDLVVAGPGAGSKLKTATDLGIQVMTEDEWLEL
VAG