Protein Info for CCNA_01549 in Caulobacter crescentus NA1000

Annotation: sulfate adenylyltransferase subunit 1/adenylylsulfate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 23 to 211 (189 residues), 143.8 bits, E=9.6e-46
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 25 to 430 (406 residues), 696.8 bits, E=9.5e-214
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 444 to 623 (180 residues), 259.9 bits, E=1.1e-81
• PF01583: APS_kinase
  • amino acids 459 to 612 (154 residues), 244.8 bits, E=7.6e-77

Best Hits

Swiss-Prot: 64% identical to CYSNC_XYLFA: Bifunctional enzyme CysN/CysC (cysNC) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 100% identity to ccr:CC_1482)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Sequence Analysis Tools

See A0A0H3C9Q7 at UniProt or InterPro

Protein Sequence (635 amino acids)

>CCNA_01549 sulfate adenylyltransferase subunit 1/adenylylsulfate kinase (Caulobacter crescentus NA1000)
MAHQSALIAEDIEAYLHQHQHKSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQMAALE
VDSRKVGTQGGAIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRN
MVTGASTADAAVILIDARKGVLTQTRRHSYLVSLLGIRHVVLAVNKMDLVGWDKGVFDAI
VADYRAFAEQIGLSVFTPIPISGLGGDNMATRSDATPWFEGPILMDWLEAVEVEDDLQAK
PFRMPVQWVNRPNLDFRGFSGLIASGSIKPGDRIRALPSGRESRVARIVTLPGDLDQAVA
GQSVTLTLEDEIDISRGDVIAAADAPAPVANQFEATLVWMDDEPLPPGRTYLLKLGARTV
GASITDIKHRVNVNTLEKTAAKRLELNEIGVCNLSLDQAIPFEAYAENRHMGGFILIDRL
SNRTVGAGLINFALRRADNIHWQHTDVTKVSRAALKRQRGQVVWLTGLSGAGKSTIANLV
EKRLHALGRHTYLLDGDNVRHGLNKDLGFTEEDRVENIRRVAEVAKLMVDAGLIVLTAFI
SPFRAERQLARDLLEPGEFIEVFVDTPLAVAEARDVKGLYKKARSGQLKNFTGVDSPYEA
PESPELRIDTTAIDPVEAAERIVAWLEGQEIDYTI