Protein Info for CCNA_01547 in Caulobacter crescentus NA1000

Annotation: methionyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 TIGR00398: methionine--tRNA ligase" amino acids 4 to 553 (550 residues), 447.6 bits, see alignment E=3.2e-138 PF09334: tRNA-synt_1g" amino acids 4 to 405 (402 residues), 461.6 bits, see alignment E=3e-142 PF00133: tRNA-synt_1" amino acids 8 to 242 (235 residues), 27 bits, see alignment E=2.9e-10 PF19303: Anticodon_3" amino acids 417 to 568 (152 residues), 204 bits, see alignment E=1.8e-64

Best Hits

Swiss-Prot: 100% identical to SYM_CAUVC: Methionine--tRNA ligase (metG) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to ccr:CC_1480)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8H5U4 at UniProt or InterPro

Protein Sequence (569 amino acids)

>CCNA_01547 methionyl-tRNA synthetase (Caulobacter crescentus NA1000)
MARILITSALPYINGIKHLGNLAGSMLPADVYARFKRAQGHETLYICATDEHGTPAELAA
AAAGQDVATYCAEQHVLQHEVGRAFGLSWDHFGRSSSPQNHRLTQHFCQALEDHGLIEER
VDQMVYSVDDKRFLPDRYVEGTCPHCKFEKARGDQCDNCGNLLDPTDLIDPYSVISGSRN
IEVRDTKHLYLLQTKMQDKIRAWVDAHADWPPLARSIAYKHLDEGLIDRGITRDLAWGIP
VAQDGVPRPGFEEKVFYVWFDAPIEYIAATQEWAEGSPDRDWKRWWRTDAGADDVRYVQF
MGKDNVAFHTVSFPATILGSEEPWKSVDMLKAFNWLNWYGGKFSTSNKRGVFMDAALEIL
PPDLWRWYLTANSPEGSDTAFTWEQFASAVNRDLADVLGNFVNRILKFNESKFEGVVPAG
GEPGPLEEKLFADVSARLADLAEQMDAIEIRKSAQALRALWVVGNEYLQEAAPWTAIKTD
RDRAAVIVRTALNLAALYAKISAPFIPFAAEKIGDAFGLDFPASWPSNDAKAELNTLSVG
RPITVPEVLFKKIEDEQIAEWTARFGGAE