Protein Info for CCNA_01414 in Caulobacter crescentus NA1000

Annotation: 3-hydroxyisobutyryl-CoA hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 12 to 186 (175 residues), 104.4 bits, E=6.7e-34
• PF16113: ECH_2
  • amino acids 15 to 337 (323 residues), 406.5 bits, E=1.2e-125

Best Hits

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to ccr:CC_1352)

Predicted SEED Role

"3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 3.1.2.4)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• adipate degradation (5/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (4/5 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• β-alanine biosynthesis II (4/6 steps found)
• acrylate degradation I (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• valproate β-oxidation (5/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• phenylacetate degradation I (aerobic) (4/9 steps found)
• superpathway of phenylethylamine degradation (5/11 steps found)
• 3-phenylpropanoate degradation (4/10 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 3.1.2.4 or 4.2.1.17

Sequence Analysis Tools

See A0A0H3C7V7 at UniProt or InterPro

Protein Sequence (350 amino acids)

>CCNA_01414 3-hydroxyisobutyryl-CoA hydrolase (Caulobacter crescentus NA1000)
MTDDPEVLIRVEKNVGRITLNRPKALHALTLGMCETMIGALLDWQDDPEVYMVLIDHTGE
RGFCAGGDIRMLADSGAKDGVEARRFFHTEYQLNHLLFTYETPVVAVMDGIVMGGGVGIS
MPAHIRIATERTTFAMPETGIGLFPDVGGGWYLPRLPGKAGLWLALTGARIKGADCMRLG
IATHFVEFGAVEGLKKAIIADPRRIEETLRKYRADAGKASLLGFEQDLNRLFVGESVEEI
FEFLTLDSSDWGKAQLEVMKTKSPQTLKVAFEQLKRGAAMEDFADNMAMEYRIGSRVVMK
HDFIEGVRAVIVDKDNAPRWSPARVEDVTDAALAEIFAPLPPGEEWTPLT