Protein Info for CCNA_01123 in Caulobacter crescentus NA1000

Annotation: peptide chain release factor RF-3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 TIGR00503: peptide chain release factor 3" amino acids 7 to 532 (526 residues), 622.9 bits, see alignment E=4e-191 PF00009: GTP_EFTU" amino acids 11 to 278 (268 residues), 175.1 bits, see alignment E=2.4e-55 TIGR00231: small GTP-binding protein domain" amino acids 15 to 147 (133 residues), 78.1 bits, see alignment E=6.6e-26 PF01926: MMR_HSR1" amino acids 16 to 142 (127 residues), 28.8 bits, see alignment E=2.3e-10 PF22042: EF-G_D2" amino acids 301 to 386 (86 residues), 73.1 bits, see alignment E=3.3e-24 PF16658: RF3_C" amino acids 392 to 518 (127 residues), 142.1 bits, see alignment E=1.9e-45

Best Hits

Swiss-Prot: 52% identical to RF3_CUPNJ: Peptide chain release factor 3 (prfC) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 100% identity to ccr:CC_1070)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C769 at UniProt or InterPro

Protein Sequence (534 amino acids)

>CCNA_01123 peptide chain release factor RF-3 (Caulobacter crescentus NA1000)
MSTAPAAEAARRRTFAIISHPDAGKTTLTENILLAGGAIRAAGAVRARGENRRTRSDWMK
IERERGISVSASVMTFDHDGKMFNLLDTPGHEDFSEDTYRTLTAADAAIMVLDAAKGIEP
QTLKLFEVCRLRDIPIITFINKMDREAQDPIELLDEIASKLALDPAPLYWPAGSGGRFKG
MLDMREGMFIPYAKRTGGAADDEGHPEPVAFAGNSINQFLEDAEKAELEEQAELVGGGYP
TFEVQSFLEGHMTPVFFGSALRHFGVKQLLAGIGAYAPSPKAMKAEKNGEPTSVEPGDKE
VSGFVFKVQANMDPNHRDRIAMLRLTSGRFTRGMKLKAQNTGKAMSVNAPIMFFASDREL
AEDAFAGDVIGIPNHGVLRVGDSLSESGTLRFAGLPNFAPEILRRVRVKDPLKAKHLKKA
LEGLAEEGVTQLFRPMIGSDFIVGAVGQLQFEVMADRLSNEYQLDVIFENSPFAEARWLV
GERAAVEDFVDKHKSAMGQDIDEAPVFLGKSAWEIGYVADKYPKVRFERTKERA