Protein Info for CCNA_00851 in Caulobacter crescentus NA1000

Annotation: periplasmic multidrug efflux lipoprotein precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 45 to 375 (331 residues), 279.8 bits, see alignment E=1.2e-87 PF16576: HlyD_D23" amino acids 67 to 294 (228 residues), 62.5 bits, see alignment E=7.3e-21 PF13533: Biotin_lipoyl_2" amino acids 68 to 115 (48 residues), 32.7 bits, see alignment 1e-11 PF00529: CusB_dom_1" amino acids 162 to 361 (200 residues), 34.8 bits, see alignment E=2.7e-12 PF13437: HlyD_3" amino acids 178 to 291 (114 residues), 36.6 bits, see alignment E=1.3e-12

Best Hits

Swiss-Prot: 54% identical to ACRA_ECOLI: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli (strain K12)

KEGG orthology group: K03585, membrane fusion protein (inferred from 100% identity to ccr:CC_0808)

MetaCyc: 54% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C5Q7 at UniProt or InterPro

Protein Sequence (388 amino acids)

>CCNA_00851 periplasmic multidrug efflux lipoprotein precursor (Caulobacter crescentus NA1000)
MHFQRSVAATTVGLAAIALTLSACGQKPGGGMGMGMGGPTEVGYIVAQSQSVGLSTELAG
RTSAYLVSEVRPQVSGVIQKRLFEEGAVVRAGQPLYQIDPATYQAAYNSAAAALAQAQAQ
ATAAKLKADRYKALVETGAVSRQDNDDAQAAALQTAAAVGVQKAALDSARINLNYARVTA
PISGRIGKSSVTAGALVTANQATALATVQDLSKVYVDLTQTSAELLKLQAQFASGKVGRS
GSAQVTLKLEDGSTYPIPGRLEFSDITVDPGTGAVGLRAVFDNPKGVLLPGMYVRAVLSQ
GVAPSGILIPQTAVNRDPKGGATVMLVGAKGPEPRPVTLGQTVGDKWLVTSGLNAGDKVI
VEGLMKVRPGAPIKAVPAGAAPAAQAQR