Protein Info for CCNA_00496 in Caulobacter crescentus NA1000

Annotation: threonyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 655 PF02824: TGS" amino acids 4 to 51 (48 residues), 31.5 bits, see alignment 3e-11 TIGR00418: threonine--tRNA ligase" amino acids 77 to 637 (561 residues), 691.4 bits, see alignment E=6.1e-212 PF07973: tRNA_SAD" amino acids 174 to 224 (51 residues), 57.1 bits, see alignment 2.8e-19 PF00587: tRNA-synt_2b" amino acids 323 to 535 (213 residues), 166.4 bits, see alignment E=1.4e-52 PF03129: HGTP_anticodon" amino acids 547 to 636 (90 residues), 73.2 bits, see alignment E=3.1e-24

Best Hits

Swiss-Prot: 100% identical to SYT_CAUVN: Threonine--tRNA ligase (thrS) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to ccs:CCNA_00496)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GZS7 at UniProt or InterPro

Protein Sequence (655 amino acids)

>CCNA_00496 threonyl-tRNA synthetase (Caulobacter crescentus NA1000)
MIDLVFPDGSSRQYPDGATGRDVAAAISKSLEKKALLIKLDGQVLDLDRRLTPDLLTGER
KFEILTREAPEALDTIRHDTAHVLAEAVQELFPGTQVTIGPNVEDGFYYDFARDEPFSLD
DLEKIEKRMKEIVDRDEKITREVWDRNEAIAHFDGIGEQYKAQIIRDLPDTDTITVYRQG
NWKDLCRGPHLPSTKHVGKAFKLTKLAGAYWRGDQNNAQLQRIYGTAWASEADLEAHLKR
IEEAEKRDHRKLGKTMDLFHIQEEGKGMVFWHPKGWALYRVLEDYMRRRLDAAGYKEVKT
PQILDRSLWEKSGHAEKFGHAMFMCESAEGEVLAVKPMNCPGHIQIFNVGQKSYRELPLR
MAEFGACHRYEPSGAMHGIMRVRAFTQDDAHIFCREEQVTEESARFIELLRSVYNDLGMT
LADTKFSTRPELRAGTDETWDKAEAALAAAAEAAGETLTLQPGEGAFYGPKLEFSLKDAI
GRVWQCGTLQLDFVLPERLDAEYVSEDGSKKRPVMLHRAILGSFERFIGILLENFAGALP
VWLAPTQVVVATITSDADDYAREVVEKLTKLGMRAELDLRNEKINYKIREHSLAKVPVIA
VVGRKEAETGQLALRRLGGEGQSVLSLEEALRVLKSDATPPDVARALAVQEAVTA