Protein Info for CCNA_00457 in Caulobacter crescentus NA1000

Annotation: phosphoenolpyruvate-protein phosphotransferase/phosphocarrier protein HPr/PTS system, glucose-specific IIA subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 852 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 470 to 488 (19 residues), see Phobius details TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 8 to 128 (121 residues), 130.1 bits, see alignment E=6.5e-42 PF00358: PTS_EIIA_1" amino acids 8 to 130 (123 residues), 145.4 bits, see alignment E=1.9e-46 TIGR01003: phosphocarrier, HPr family" amino acids 171 to 250 (80 residues), 70.2 bits, see alignment E=1.7e-23 PF00381: PTS-HPr" amino acids 171 to 250 (80 residues), 88.3 bits, see alignment E=7.1e-29 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 290 to 839 (550 residues), 466.2 bits, see alignment E=2.1e-143 PF05524: PEP-utilisers_N" amino acids 291 to 406 (116 residues), 76 bits, see alignment E=7e-25 PF00391: PEP-utilizers" amino acids 432 to 504 (73 residues), 68.9 bits, see alignment E=6.3e-23 PF02896: PEP-utilizers_C" amino acids 531 to 814 (284 residues), 340.5 bits, see alignment E=1.9e-105

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to ccs:CCNA_00457)

Predicted SEED Role

"PTS system, glucose-specific IIA component / Phosphocarrier protein of PTS system / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Mannitol Utilization or Fructose utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C4Q4 at UniProt or InterPro

Protein Sequence (852 amino acids)

>CCNA_00457 phosphoenolpyruvate-protein phosphotransferase/phosphocarrier protein HPr/PTS system, glucose-specific IIA subunit (Caulobacter crescentus NA1000)
MIVSNLVLTAPLQGWVSALEEAPDAVFAERMLGDGLAIDPLGSTLHAPCDGSVVSVHRAR
HAVTLRATNGAEILMHVGLETVALNGEGFEVFVEEGQTVKAGDRLIGLDLDLLAQRARSL
ITPVVITNGEAFQIVRRDQDRQIGVGQFLMELSPIAGASRAAASGAADQITRQIIVPLAH
GIHARPAARIAQMAKTFASDLTLATGPRRANARSPVGLMSLAIRHGDTIQLLASGPDAQA
AVTALAELIEGGMGEGAPVAAKTSSTKTAPTKTEPSEPPPPAPLPRDGVLKGVPAAPGLA
IGKAVRLSTAEIVVREAGEGVAHEEAALAVALETVRARIGKAAETGDKARKAILAAHQAF
LDDPELYAGAHRLIAEGKSAGFAWRRAVGGYVQALQALGDRRMAERVDDLIDLERQVLRA
LSGEEETGAALAPGSILLADELLPSQLMGADPAALAGFATARGGPTSHVAILAAAMGVPA
LVAVGGALSKVKDGATLILDADAGTLRVAPDAKALEAAQTALAQRQQRKAAAKAAAHEPA
VTRDGVRIEVFANTGSVADAQAAVANGAEGSGLLRTEFLFLDRETPPDEDEQARQYQAIA
EALDGRPLIIRTLDVGGDKAAPYLPIPAEENPALGLRGVRVSLWRPHLLKAQLRAILRVE
PRGQCKIMVPMVASLDELRAVRAVLEEAKRELGITDRVQLGVMIETPAAAVTADLLAAEA
DFLSIGTNDLTQYVLAMDRGNPELAAGIDALHPAVLRMIDQTCQGAARHHRWVGVCGGLA
SDLDATPILLGLGVAELSTTASIAPDVKARVRALSLEACRALAAQALEQTSPQAVRALVQ
GQTNTPLGGLNP