Protein Info for CCNA_00212 in Caulobacter crescentus NA1000
Annotation: N-carbamoylputrescine amidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to AGUB_SOLLC: N-carbamoylputrescine amidase (CPA) from Solanum lycopersicum
KEGG orthology group: K12251, N-carbamoylputrescine amidase [EC: 3.5.1.53] (inferred from 100% identity to ccr:CC_0212)MetaCyc: 52% identical to citrullinase (Francisella tularensis)
Citrullinase. [EC: 3.5.1.20]
Predicted SEED Role
"N-carbamoylputrescine amidase (3.5.1.53) / Aliphatic amidase AmiE (EC 3.5.1.4)" in subsystem Arginine and Ornithine Degradation or Polyamine Metabolism (EC 3.5.1.4)
MetaCyc Pathways
- uracil degradation III (5/5 steps found)
- L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) (3/3 steps found)
- L-citrulline degradation (3/3 steps found)
- putrescine biosynthesis II (3/3 steps found)
- urea degradation I (3/3 steps found)
- indole-3-acetate biosynthesis III (bacteria) (2/2 steps found)
- L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
- cyanate degradation (2/3 steps found)
- acrylonitrile degradation I (1/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (1/2 steps found)
- cyanuric acid degradation II (3/5 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (1/3 steps found)
- superpathway of acrylonitrile degradation (1/3 steps found)
- superpathway of allantoin degradation in yeast (3/6 steps found)
- cyanuric acid degradation I (2/5 steps found)
- superpathway of polyamine biosynthesis II (4/8 steps found)
- superpathway of atrazine degradation (3/8 steps found)
- allantoin degradation IV (anaerobic) (3/9 steps found)
- indole-3-acetate biosynthesis II (3/12 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.4, 3.5.1.53
Use Curated BLAST to search for 3.5.1.20 or 3.5.1.4 or 3.5.1.53
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0H3C689 at UniProt or InterPro
Protein Sequence (292 amino acids)
>CCNA_00212 N-carbamoylputrescine amidase (Caulobacter crescentus NA1000) MARTLSVAAIQTSYGMDLQANIKKTEGFIREAASKGAQVILPSELFQGPYFCVAQEERWF AQAHPWREHPVVKAIAPLAGELGVVIPISIFEREGPHYFNSLVMADADGSLMGVYRKSHI PDGPGYMEKYYFRPGDTGFKVWDTRFGRIGVGICWDQWYPECARAMALMGAEALFYPTAI GSEPHDASLDTALPWRRAMQGHAVSNVIPVIGANRIGFEPWDGYPNGGQTFYGSSFVADH RGDLVSELGRADEGLVSATFDLDFLTTHRAAWGFFRDRRPELYTALSSGRPA