Protein Info for CCNA_00159 in Caulobacter crescentus NA1000

Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 821 TIGR01059: DNA gyrase, B subunit" amino acids 26 to 818 (793 residues), 969.2 bits, see alignment E=5.2e-296 PF02518: HATPase_c" amino acids 53 to 152 (100 residues), 62.6 bits, see alignment E=1.2e-20 PF00204: DNA_gyraseB" amino acids 254 to 423 (170 residues), 191.6 bits, see alignment E=2e-60 PF01751: Toprim" amino acids 452 to 564 (113 residues), 67.3 bits, see alignment E=2.9e-22 PF21249: GyrB_hook" amino acids 566 to 587 (22 residues), 23.9 bits, see alignment (E = 6.2e-09) PF00986: DNA_gyraseB_C" amino acids 750 to 811 (62 residues), 99.6 bits, see alignment 2.3e-32

Best Hits

Swiss-Prot: 100% identical to GYRB_CAUVN: DNA gyrase subunit B (gyrB) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 100% identity to ccs:CCNA_00159)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GXQ0 at UniProt or InterPro

Protein Sequence (821 amino acids)

>CCNA_00159 DNA gyrase subunit B (Caulobacter crescentus NA1000)
MTENTEDQVPDLSTPEMTTEEAAAQYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHM
VYEVVDNAIDEALAGHATKVQVILNADGSVTVTDDGRGIPVDMHEGEGVSAAEVIMTQLH
AGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELLIHRNGKVHQMRFERGDAVTSLKVTGD
SPVRTEGPKAGETLTGTEVTFFPSKDTFAFIEFDRKTLEHRLRELAFLNSGVTIWFKDHR
DVEPWEEKLFYEGGIEAFVRHLDKAKTPLLKAPIAVKGVKDKVEIDLALWWNDSYHEQML
CFTNNIPQRDGGTHLSAFRAALTRIITSYAESSGILKKEKVSLGGEDSREGLTCVLSVKV
PDPKFSSQTKDKLVSSEVRPAVEGLVSEGLSTWFEEHPNEAKAIVTKIAEAAAAREAARK
ARELTRRKSALDITSLPGKLADCSERDPAKSEIFIVEGDSAGGSAKQARNRDNQAVLPLR
GKILNVERARFDKMLSSDQIGTLITALGAGIGRDDFNPDKVRYHKIVLMTDADVDGAHIR
TLLLTFFYRQMPELIERGYIYIAQPPLYKASKGKSSRYLKDDAEMDAFLVDEGVDGAELD
LASGERMTGQDLLALVQTCRSAKANIDRLAARAPATAIEQAALSGLLGESPNAAAAATRL
DLYAEEGDGPWSGERGDTGFVFSRVRRGVSERVVLDDVLLHAADARRLAERAVKLTEIFS
GRAIFRRKDKSTTVRGPLDLVNAVLDAGRKGLTIQRYKGLGEMNPDQLWETTLDAEARTL
LQVRVNHADDADDMFSRLMGDLVEPRREFIQENALDAEVDV