Protein Info for CCNA_00158 in Caulobacter crescentus NA1000

Annotation: DNA replication and repair protein recF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF02463: SMC_N" amino acids 7 to 374 (368 residues), 63 bits, see alignment E=2.9e-21

Best Hits

Swiss-Prot: 100% identical to RECF_CAUVN: DNA replication and repair protein RecF (recF) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K03629, DNA replication and repair protein RecF (inferred from 100% identity to ccr:CC_0159)

Predicted SEED Role

"DNA recombination and repair protein RecF" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GXP9 at UniProt or InterPro

Protein Sequence (387 amino acids)

>CCNA_00158 DNA replication and repair protein recF (Caulobacter crescentus NA1000)
MASAALLSLTLADFRSYERARLETGGRSVYLFGANGAGKTNLLEAISLLSPGKGLRGVSL
AEVGRRLPGEAVGRAWAVAAEVQSGEDAPVRIGTGVEQGGAARRTVRLEGETVPPGRLAD
HVRPIWLTPAQDRLFLEAASERRRFFDRLVFAGEPAHAANANGYDKAQRERMRLLVDAAE
TGAPADAAWLTALEARLAEFGALLAQARARTLLALQAEIDGRGDRPFPLARLGLTGEWER
MALEGAPFAEIELKLAQALASARARDGAAGRALTGPHRGDLAIFHVEKDRPAAECSTGEQ
KALILNLVLAQAARLSRAESAPNPVILLDEVAAHLDLTRRAALADELTALKLQAFLTGTD
ESLFDHLKGRALGVRVGDAGLTTLEDE