Protein Info for CCNA_00069 in Caulobacter crescentus NA1000

Annotation: O-sialoglycoprotein endopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 11 to 329 (319 residues), 385.8 bits, see alignment E=1.4e-119 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 12 to 322 (311 residues), 292.9 bits, see alignment E=2.9e-91 PF00814: TsaD" amino acids 35 to 322 (288 residues), 285.4 bits, see alignment E=2.8e-89

Best Hits

Swiss-Prot: 100% identical to TSAD_CAUVC: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 100% identity to ccs:CCNA_00069)

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C563 at UniProt or InterPro

Protein Sequence (367 amino acids)

>CCNA_00069 O-sialoglycoprotein endopeptidase (Caulobacter crescentus NA1000)
MTSPKQSDLLILGLETSCDETAASVVRRAADGTVTVLSSVIGTQFEKHAPFGGVVPEIAA
RAHVESIDAIAAEAVRAAGVGFGDLDGVAATAGPGLVGGVMVGLAFGKAVALARGAPLVA
VNHLEGHAVSARLGADIAYPFLLLLVSGGHCQLLEVSGVGACKRLGTTIDDAAGEAFDKI
AKSLGLPYPGGPALEKLAVGGDPTRYALPRALLGRKDCDFSFSGLKTAAARIAETLTTDD
ARRDLAAGVQAAIARQLSERVDRAMKLYKDSHDPEDLRFVVAGGVAANGAVRAALLADCE
KNGFSFAAPPLAYCTDNAAMIALAGAERLALGIFDDLDAIARPRWPLDEAAALANPANAY
GRKGAKA