Protein Info for CA265_RS24660 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF00128: Alpha-amylase" amino acids 59 to 431 (373 residues), 86.3 bits, see alignment E=3.1e-28

Best Hits

KEGG orthology group: None (inferred from 59% identity to dfe:Dfer_2758)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.1

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9ZCS3 at UniProt or InterPro

Protein Sequence (584 amino acids)

>CA265_RS24660 alpha-amylase (Pedobacter sp. GW460-11-11-14-LB5)
MIISLSLTFAILSLSSFAQKQSTKMNDKQIVIYQLLPRLFGNKNTTNTPYGTIEQNGSGK
FNDITDKALDGIKELHVNYVWYTGALAHASLTDYSAYGIKPDDADVVKGRAGSPYAIRDY
YDVDPDLAVDVKNRMKEFEALVKRTHAKNLKVLIDFVPNHVARSYHSYAKPKDVVDFGAQ
DDVTKAFSASNDFYYSPGQPFASPTGGATQTPLSVLQDGKFNENPAKATGNNVFSPQPKY
DDWYETIKLNYGVDYQNGEKQYFNPIPPVWLKMRDILTYWTNKGVDGFRCDMAEMVPIAF
WNWVIPQVKKVNPDLIFLGEAYNPKVYKQYLDEGKFDYLYDKVGLYDGLKKLIRNEPTAD
VAAIKHVWQVESAGFGKHMLRFLENHDEERIASAGFAGKAELALPAMVVSATLGAGPVML
YFGQEVGEPGKGQEGFGGDDNRTTIFDYWGVPSHQKWMNNGAFDGKKLSENEQKLRAYYQ
QLLKVTSSSDAVMNGEIYEVPVTGNMNNRMYAFIRYSGKQRLLVVANFDRTQTLTANIEI
PDQILKVKHSSPVTDLLTDRKLNIPAGTSIPVSLAPVSAQVIEF