Protein Info for CA265_RS24645 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 30 to 49 (20 residues), see Phobius details PF09087: Cyc-maltodext_N" amino acids 54 to 141 (88 residues), 107.4 bits, see alignment E=5.8e-35 PF00128: Alpha-amylase" amino acids 193 to 510 (318 residues), 179.1 bits, see alignment E=2.8e-56 PF10438: Cyc-maltodext_C" amino acids 566 to 641 (76 residues), 81.2 bits, see alignment E=6.7e-27

Best Hits

KEGG orthology group: None (inferred from 55% identity to cpi:Cpin_5089)

Predicted SEED Role

"Neopullulanase (EC 3.2.1.135)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.135)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9ZBN9 at UniProt or InterPro

Protein Sequence (644 amino acids)

>CA265_RS24645 alpha-amylase (Pedobacter sp. GW460-11-11-14-LB5)
MIKSLTSATFSSVIASPFNAQTKIHANVPGLKKIFIVLVVLFSSINLFAQKLERVEPMFW
YVGMHNPKLQLLVHGDNIAQTTVALTYPGVKLVKVNKVENPNYLFLDLTIAATVKSGSFP
INFSVNGKKIFSYTYELKNRDKSAGRIQGVTNKDFIYLLMPDRFSNGDKSNDVVQGLTEM
ALNRDSMYYRHGGDIQGVINHLDYLKDLGVTTVWMTPEVENDMPQASYHGYAVTDHYKID
PRYGTNALYKKYVEIAHAKGLKVIKDIVHNHIGTQHWFYKDLPMKNWLNQWPKYTQTSYR
DQTVMDVHASTADRKQMLDGWFVPSMPDLNQTNPYVQNYLTQNHIWWIEYAGIDGLRLDT
YGYNDPVYMADWALKVQAEFPHLSVFGETLVTAVANQAFFTGGNTVNRGFDTHLQGITDA
TLKDAIYEGINGKNGWVDGINRLYATLAHDFLYKNPNTNCIFLDNHDMSRFYSMVGEDLD
KYKMGMSILLTMRGIPEMYYGTEILMKNFSNPDGLIRSDFPGGWEGDKKDKFIANGRTNK
ENDAFNFVKTLANFRKSSAALQTGKLMQFVPQDDIYVYFRYNAEPKGTVMVIVNNIDKEK
IINTDRFAERTKGITAAKNVITGENIDFKSIKVPAKTTLVLVLN