Protein Info for CA265_RS23445 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: methylenetetrahydrofolate reductase [NAD(P)H]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02219: MTHFR
  • amino acids 4 to 315 (312 residues), 239.7 bits, E=2.2e-75
• TIGR00676: methylenetetrahydrofolate reductase [NAD(P)H]
  • amino acids 16 to 316 (301 residues), 297.1 bits, E=6e-93

Best Hits

KEGG orthology group: K00297, methylenetetrahydrofolate reductase (NADPH) [EC: 1.5.1.20] (inferred from 80% identity to psn:Pedsa_3414)

Predicted SEED Role

"5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)" in subsystem Methionine Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.20)

MetaCyc Pathways

• folate transformations I (8/13 steps found)

KEGG Metabolic Maps

• Methane metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.20

Sequence Analysis Tools

See A0A1X9ZBA5 at UniProt or InterPro

Protein Sequence (317 amino acids)

>CA265_RS23445 methylenetetrahydrofolate reductase [NAD(P)H] (Pedobacter sp. GW460-11-11-14-LB5)
MKITDHIKNAKGKTLFSFELLPPVKGQSIQWIYDAIEPLLEFNPPFIDVTSLREDYIYKE
QANGLLQKVSYRKRPGTIAICAAIIHKYKVDAVPHLICGGFTKEETENALIDLQFLGIDN
VLALRGDARKADGNFVPTPGGHCYATDLIEHIKNHNEGKFLHEDVETFKSDFCIGVAGYP
EKHFEAPNMSTDFKYLKKKVEAGAEFIVTQMFFDNQKYFDFVKKCRENDINVPIIPGLKP
ISTLSQLNVLPKIFHIDLPDELTDALSHAKNNNEAKEIGTEAMIKQCKELIDFGAPVLHF
YTMGRPEQTKKIAKAIF