Protein Info for CA265_RS22270 in Pedobacter sp. GW460-11-11-14-LB5

Updated annotation (from data): Histidine ammonia-lyase (EC 4.3.1.3)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (HISTIDINE-AMMONIA-LYASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01225: histidine ammonia-lyase
  • amino acids 13 to 505 (493 residues), 600.1 bits, E=1.5e-184
• PF00221: Lyase_aromatic
  • amino acids 15 to 471 (457 residues), 607.9 bits, E=5.9e-187

Best Hits

Swiss-Prot: 44% identical to HUTH_MYXXD: Histidine ammonia-lyase (hutH) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 76% identity to phe:Phep_1335)

MetaCyc: 40% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

• L-histidine degradation VI (6/8 steps found)
• L-histidine degradation I (3/4 steps found)
• L-histidine degradation III (3/6 steps found)
• L-histidine degradation II (2/5 steps found)

KEGG Metabolic Maps

• Histidine metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

See A0A1X9ZBQ5 at UniProt or InterPro

Protein Sequence (511 amino acids)

>CA265_RS22270 Histidine ammonia-lyase (EC 4.3.1.3) (Pedobacter sp. GW460-11-11-14-LB5)
MSEQQIFNYGTDHLTAKLALAISNGQIKGVLSQSTRDKVLESSQVVERIAVSGKAVYGIN
TGFGPLCTSMISAIDTRKLQENILKSHAVGVGEPIDSEISKLMLVLKLQALAQGYSGIKI
ETLDRMIWFLEIGATPVVPKQGSVGASGDLAPLSHLFLPLIGLGKLHYKGEIIATAQLLQ
EYQMSPLQLGPKEGLALINGTQFIAAHAVKVVQRLENVLDSADIIAAMMIEGLQGSEKPF
HAQLHQLRPYPANIAVAEQVRKLLQGSEIMKSHADCAKVQDPYSLRCIPQVHGASRTAWL
HLKEALEIELNSVTDNPVIFNDDLTISGGNFHGQPLALPLDYACLAASEIGNISDRRIYL
SLEGNTPGVPKLLMKETGLNSGFMIVQYTSAALASENKGLCFPASADSIPTSLGQEDHVS
MGSISGRKALQVIENVEKILGIELFCAAQAVDYHHPLKPGKILAAVHDFVRTEIDHFEED
QIMYDRMENAIQMVQQGKIVAVAAEAESTLT