Protein Info for CA265_RS21455 in Pedobacter sp. GW460-11-11-14-LB5

Updated annotation (from data): Formiminoglutamase (EC 3.5.3.8)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (FORMIMINOGLUTAMASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: arginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00491: Arginase
  • amino acids 53 to 236 (184 residues), 64.5 bits, E=5.8e-22

Best Hits

KEGG orthology group: None (inferred from 60% identity to phe:Phep_4273)

Predicted SEED Role

"Formiminoglutamase (EC 3.5.3.8)" in subsystem Histidine Degradation (EC 3.5.3.8)

MetaCyc Pathways

• L-histidine degradation I (3/4 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.3.8

Sequence Analysis Tools

See A0A1X9ZAU2 at UniProt or InterPro

Protein Sequence (326 amino acids)

>CA265_RS21455 Formiminoglutamase (EC 3.5.3.8) (Pedobacter sp. GW460-11-11-14-LB5)
MDNLKIYSQGDIDHLIITRDGETKLGERVNVYSGDSSSTAGISVEGLKASSAKFVLLGIP
EDIGVRANLGLAGAASMWKPGLVAFLNTQSNRFLSGEEVLVLGHFEIDEPEDSSLKGLRN
KVAQIDDLVYPVIEKIVATGKIPVVIGGGHNNAYPMIKGTSLAHKRPITVLNVDAHADLR
ELEGRHSGNGFSYALKENYLNNYLMYGLHQNYNNEAILNQIDTNPKLKAVFFDDILTGAD
FTNLVNEIGSVAGLEIDLDCVQNVLSSAETPSGFAVNDIRKLILTSAKKFSYLHLSEGAT
RMLDGRVSKLTSKLVAYLVSDFIKAH