Protein Info for CA265_RS21340 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: secretion protein HlyD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 52 to 94 (43 residues), 32.4 bits, see alignment 1.5e-11 PF00529: CusB_dom_1" amino acids 134 to 356 (223 residues), 36 bits, see alignment E=1.4e-12 PF16576: HlyD_D23" amino acids 176 to 306 (131 residues), 66.4 bits, see alignment E=5.8e-22 PF13437: HlyD_3" amino acids 230 to 348 (119 residues), 67.3 bits, see alignment E=4.8e-22

Best Hits

Swiss-Prot: 35% identical to EMRA_ACIBT: Colistin resistance protein EmrA (emrA) from Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 78% identity to phe:Phep_4261)

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9ZAL3 at UniProt or InterPro

Protein Sequence (359 amino acids)

>CA265_RS21340 secretion protein HlyD (Pedobacter sp. GW460-11-11-14-LB5)
MTTEKKKKNIVVPIILGVLLVIGIIFGITEWNYYSKHVDTDDAQIDGDISPVVARVGGYV
KDINFEENTHVTEGQILVKLDDNDYKVKLEQAQSGQKGASAGVGVAESQIVATQANTGTA
KANVDAAKSNVQAANVKVNLAQKDYNRYENLVKDGSITQQAFDQAKANKESAEAARQSAL
AAYRAAQDQYNAAVKQVGTTQSQLAVSSNVISQRQSDIDFAKLQLSYTDIKAPATGIVSK
KNVQKGQLVQAGQSLFSIVNDGSIYVTANFKETQLEKIKEGSKVEIEVDAYPDEKIHGEV
YNFSPITGAKGSLLPPDNATGNFVKVVQRVPVKIKIHPSKELLAKLRPGMSVKASVSTK