Protein Info for CA265_RS21125 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: tRNA dihydrouridine synthase DusB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 TIGR00737: putative TIM-barrel protein, nifR3 family" amino acids 5 to 324 (320 residues), 328.6 bits, see alignment E=2e-102 PF01207: Dus" amino acids 16 to 303 (288 residues), 285.4 bits, see alignment E=2.7e-89

Best Hits

KEGG orthology group: None (inferred from 84% identity to phe:Phep_0018)

Predicted SEED Role

"tRNA dihydrouridine synthase B (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9ZAH0 at UniProt or InterPro

Protein Sequence (335 amino acids)

>CA265_RS21125 tRNA dihydrouridine synthase DusB (Pedobacter sp. GW460-11-11-14-LB5)
MSVKIGNIDLGEFPLLLAPMEDVSDPPFRYVCKKNGADMMYTEFISSEGLIRDAAKSIQK
LDIFEYERPIGIQIFGGDIDHMREASEIASAAGPDLVDINYGCPVKNVVCKGAGSSLLQD
IDKMVKMTEAVVKASHLPVTVKTRLGWDDNTKNVYEVAERLQDVGIQALTIHGRTRAQLY
KGEADWSLIREIKRNPRIKIPIFGNGDVDSPEKAANWRMEYEIDGIMIGRAAIGYPWIFR
EVKHFFKTGEHLAGPTIEERIEVCRTHLDKSLEWKGPKTGIFEMRRHYANYFKGLPDFKP
YRMRLVAEPDINNIYSILEEVAEKFADYDATKVLA