Protein Info for CA265_RS18405 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 658 PF00676: E1_dh" amino acids 19 to 312 (294 residues), 238.5 bits, see alignment E=1.3e-74 PF02779: Transket_pyr" amino acids 343 to 513 (171 residues), 112.9 bits, see alignment E=2.1e-36 PF02780: Transketolase_C" amino acids 529 to 644 (116 residues), 92 bits, see alignment E=4e-30

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 75% identity to phe:Phep_3824)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) / Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z8F6 at UniProt or InterPro

Protein Sequence (658 amino acids)

>CA265_RS18405 dehydrogenase (Pedobacter sp. GW460-11-11-14-LB5)
MQFKRGDKDDKFLLNLYRRLLYPRMVEDKMLKLLRQGRIGKWFSGIGQEAIAVGSTLAMQ
SDEYILPMHRNLGVFTSRDIPLKKLMAQWQGKITGFSKGRDRSFHFGTQEYKIIGMISHL
GPQMALADGIALADVLRNQPHATLVYTGEGATSEGDFHEAVNVAAVWNLPVIFLIENNGY
GLSTPKSEQFRCKNLVDKAIGYGVEGIQIDGNNILEVYDTINQLAMEIRKDPRPVLVECL
TFRMRGHEEASGTKYVPQELFDEWEKKDPLNNYEAYLIEQGVLTPDTVIDIKIQVKRDIE
LEIEEAFNEDEPIANADQEETDMYFPYRQQVIQPDESSTEKRYLDAITDGLDLAMQKYPN
LVLMGQDIADYGGAFKITDGFTAKYGRGRVRNTPICESAIVGAGLGLSINGYKAVVEMQF
ADFVTVGFNQIVNNLAKTHYRWGEKADVVVRMPTGAGTGAGPFHSQSNEAWFTKTPGLKI
VYPAFPEDAKGLLLAAIEDPNPVLYFEHKYLYRSLSAPVPDGYYTTEIGKAVRLAEGDKF
TIITYGLGVHWALDYMEQYPESGATLIDLRTLQPWDKETVSAAVKATGRVLILHEDTLTN
GFGAELSAWIGEHCFAYLDAPVMRCASLDTAIPMSKILEEDFLAKARLAETIDKLLKY