Protein Info for CA265_RS16745 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 TIGR02350: chaperone protein DnaK" amino acids 3 to 598 (596 residues), 984.2 bits, see alignment E=1.1e-300 PF00012: HSP70" amino acids 4 to 598 (595 residues), 868.9 bits, see alignment E=1.7e-265 PF06723: MreB_Mbl" amino acids 115 to 370 (256 residues), 59.1 bits, see alignment E=3.4e-20

Best Hits

Swiss-Prot: 77% identical to DNAK_PARD8: Chaperone protein DnaK (dnaK) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 91% identity to phe:Phep_0633)

MetaCyc: 61% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9ZCW2 at UniProt or InterPro

Protein Sequence (635 amino acids)

>CA265_RS16745 molecular chaperone DnaK (Pedobacter sp. GW460-11-11-14-LB5)
MGKIIGIDLGTTNSCVSVMEGNEPVVIANSEGKRTTPSIVAFAENGERKVGEPAKRQAIT
NPTKTIYSIKRFMGNSYDESAKEAGRVPYKVIKGDNNTPRVEIDDRKYTPQEISAMILQK
MKKTAEDFLGQEVTEAVITVPAYFNDAQRQATKEAGEIAGLTVKRIINEPTAAALAYGLD
KAHKDMKIVVFDCGGGTHDVSVLELGDGVFEVKSTDGDTHLGGDDFDHIIIDWLTSEFKA
ENSMDLAKDPMALQRLKEAAEKAKIELSSTTSTEINLPYITADASGPKHLVRTLSRAKFE
QLADSLIKRTIDPCKSALKNAGLSTSDIDEIILVGGSTRIPAIQEAVKAFFGKEPSKGVN
PDEVVAIGAAIQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTKLIESNTTIPTKKSETF
STAADNQPSVEIHILQGERPMAGQNRTIGRFILDGIPPAPRGVPQVEVSFDIDANGILHV
SAKDKATGKEQKIRIEASSGLTDAEIKKMKEEAEANAADDAKQKEEADKINGADALIFST
EKQLKEFGDKLSADKKAPIEAGLEKLKAAHGSRNFADIDAAQAELQNAWNAASEEMYKDG
GQPQGGEQPQADGQPQGGDNVTDVDFEEVKEDDKK