Protein Info for CA265_RS15825 in Pedobacter sp. GW460-11-11-14-LB5
Annotation: ketol-acid reductoisomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to ILVC_CYTH3: Ketol-acid reductoisomerase (NADP(+)) (ilvC) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 93% identity to phe:Phep_3422)MetaCyc: 72% identical to ketol-acid reductoisomerase (NADP+) (Escherichia coli K-12 substr. MG1655)
Ketol-acid reductoisomerase. [EC: 1.1.1.86]; 1.1.1.86 [EC: 1.1.1.86]; 2-dehydropantoate 2-reductase. [EC: 1.1.1.86, 1.1.1.169]
Predicted SEED Role
"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of coenzyme A biosynthesis I (bacteria) (9/9 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-valine biosynthesis (4/4 steps found)
- phosphopantothenate biosynthesis I (4/4 steps found)
- pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
- phosphopantothenate biosynthesis III (archaea) (2/4 steps found)
- superpathway of L-threonine metabolism (12/18 steps found)
- L-isoleucine biosynthesis III (4/7 steps found)
- superpathway of coenzyme A biosynthesis II (plants) (5/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Pantothenate and CoA biosynthesis
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.169
Use Curated BLAST to search for 1.1.1.169 or 1.1.1.86
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9Z7M1 at UniProt or InterPro
Protein Sequence (493 amino acids)
>CA265_RS15825 ketol-acid reductoisomerase (Pedobacter sp. GW460-11-11-14-LB5) MANYFNTLPLREKLNQLGVCDFMDSSEFLDGVNALKGKKLVIVGCGAQGLNQGLNLRDSG LDVSYTLRKEAIEGKRDSWKNATENNFTVGTYEELIPNADVVINLTPDKQHTAVVNAIMP LMKEGATLLYSHGFNIVEEGMQIRKDLTVIMVAPKCPGSEVRAEYVRGFGVPTLIAVHPE NDPQGKGLAQAKAYCAGTGGHRAGVLKSSFVAEVKSDLMGEQTILCGLLQTGSILSFDKM VEKGIDAGYASKLVQYGVEVITEALKQGGITAMMDRLSNVAKIKAFEISEELKDIMRPLF QKHQDDIMSGEFSRTMMEDWANGDKNLLKWRAETGETAFEKTPAGDVKIGEQEYFDNYTL MVAFVRAGVELAFETMVQAGIKPESAYYESLHETPLIANTIARKKLFEMNRVISDTAEYG CYLFDQACKPLLGDFMKKVDTDLVGKNFNEGKDGAVDNRKLIDINEAIRSHEVEQIGATL RKAMTAMKAIKTA