Protein Info for CA265_RS15110 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: NAD(P)-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00106: adh_short" amino acids 3 to 191 (189 residues), 191 bits, see alignment E=4.8e-60 PF23441: SDR" amino acids 5 to 208 (204 residues), 28.3 bits, see alignment E=3.6e-10 PF01370: Epimerase" amino acids 5 to 124 (120 residues), 25.4 bits, see alignment E=2.8e-09 PF08659: KR" amino acids 6 to 168 (163 residues), 57.6 bits, see alignment E=5e-19 PF02719: Polysacc_synt_2" amino acids 6 to 195 (190 residues), 34.4 bits, see alignment E=4.2e-12 PF13561: adh_short_C2" amino acids 9 to 226 (218 residues), 142.7 bits, see alignment E=4.3e-45

Best Hits

Swiss-Prot: 46% identical to YDFG_ECOL6: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 79% identity to phe:Phep_0979)

MetaCyc: 46% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"oxidoreductase, short chain dehydrogenase/reductase family protein"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z6N0 at UniProt or InterPro

Protein Sequence (252 amino acids)

>CA265_RS15110 NAD(P)-dependent oxidoreductase (Pedobacter sp. GW460-11-11-14-LB5)
MSKIALITGATSGIGEACAQTFAQQGYNLILLARREDRLAKIAHHLEDKYAITIKQVFAD
VRDKESLAAALEVLPAEWKKVDVLINNAGLSQGLDPIDKGDTNDWDTMIDTNVKGLLYVT
KIVSNWMIPNQSGHIINIGSIAGKEVYPNGNVYCASKHAVDALSKGMRIDLLPHGIKVTE
INPGMVETEFSVVRFKGDEDRAKKVYENLEPLIADDIADAIWYVVSRPKHVNINDMLIMP
TAQATATIINRV