Protein Info for CA265_RS14530 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: nicotinate-nucleotide diphosphorylase (carboxylating)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00078: nicotinate-nucleotide diphosphorylase (carboxylating)
  • amino acids 10 to 278 (269 residues), 322.9 bits, E=7.2e-101
• PF02749: QRPTase_N
  • amino acids 18 to 107 (90 residues), 86.3 bits, E=1.1e-28
• PF01729: QRPTase_C
  • amino acids 109 to 278 (170 residues), 207.4 bits, E=1.3e-65

Best Hits

Swiss-Prot: 49% identical to NADC_ARATH: Nicotinate-nucleotide pyrophosphorylase [carboxylating], chloroplastic (QPT) from Arabidopsis thaliana

KEGG orthology group: K00767, nicotinate-nucleotide pyrophosphorylase (carboxylating) [EC: 2.4.2.19] (inferred from 75% identity to phe:Phep_1198)

Predicted SEED Role

"Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 2.4.2.19)

MetaCyc Pathways

• aspartate superpathway (21/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD de novo biosynthesis II (from tryptophan) (6/9 steps found)
• superpathway of NAD biosynthesis in eukaryotes (7/14 steps found)
• nicotine biosynthesis (3/9 steps found)
• superpathway of nicotine biosynthesis (4/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Nicotinate and nicotinamide metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.19

Sequence Analysis Tools

See A0A1X9Z6C9 at UniProt or InterPro

Protein Sequence (280 amino acids)

>CA265_RS14530 nicotinate-nucleotide diphosphorylase (carboxylating) (Pedobacter sp. GW460-11-11-14-LB5)
MDTQLIHQFIKNALTEDVGDGDHTSLSTIPAGTQGKAKLIIKENGVLAGIELAIEIFKEV
DAELKVDVLLQDGAEVKVGDIALTVSGSTHAILIAERLVLNCMQRMSGIATKTHRIVSLL
KTTKTKILDTRKTTPGLRYLEKWAVRIGGGVNHRIGLYDMILIKDNHVDYAGGISNAITA
AKKYLIDQNKVLQIEIEVRNLDELSQVLAIGGVDRIMLDNFSFENLRAAVKLIDGKFITE
ASGGITEENVAAYAACGVDFISMGALTHSVKSLDMSLKAY