Protein Info for CA265_RS13095 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: thymidylate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00303: Thymidylat_synt
  • amino acids 2 to 264 (263 residues), 442.4 bits, E=2.4e-137
• TIGR03284: thymidylate synthase
  • amino acids 2 to 85 (84 residues), 143.2 bits, E=4.8e-46
  • amino acids 84 to 264 (181 residues), 331.6 bits, E=2e-103

Best Hits

Swiss-Prot: 84% identical to TYSY_CYTH3: Thymidylate synthase (thyA) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K00560, thymidylate synthase [EC: 2.1.1.45] (inferred from 89% identity to phe:Phep_1422)

MetaCyc: 72% identical to thymidylate synthase (Escherichia coli K-12 substr. MG1655)
Thymidylate synthase. [EC: 2.1.1.45]

Predicted SEED Role

"Thymidylate synthase (EC 2.1.1.45)" in subsystem Folate Biosynthesis (EC 2.1.1.45)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• folate transformations III (E. coli) (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• folate transformations II (plants) (9/11 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• pyrimidine deoxyribonucleosides salvage (4/5 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)

KEGG Metabolic Maps

• One carbon pool by folate
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.45

Sequence Analysis Tools

See A0A1X9Z5M4 at UniProt or InterPro

Protein Sequence (264 amino acids)

>CA265_RS13095 thymidylate synthase (Pedobacter sp. GW460-11-11-14-LB5)
MKQYLDLMQHVLDHGAQKHDRTGTGTISVFGYQMRFNLQEGFPMVTTKKLHLKSIIHELI
WFLTGETNIKYLKDNGVRIWDEWADENGNLGPVYGSQWRSWPAPDGQHIDQITNIINTIK
NNPDSRRIIVSAWNVAEIENMALPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIAS
YALLTMMVAQVCGLEAGDFIHTLGDAHLYNNHIEQANLQLSREPKPLPTMKINPEVKSIF
DFKFEDFTLENYEAHPHIKGIVAV