Protein Info for CA265_RS10450 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 PF10396: TrmE_N" amino acids 6 to 121 (116 residues), 135.7 bits, see alignment E=3.5e-43 TIGR00450: tRNA modification GTPase TrmE" amino acids 12 to 452 (441 residues), 405.2 bits, see alignment E=4.2e-125 PF12631: MnmE_helical" amino acids 125 to 449 (325 residues), 184.1 bits, see alignment E=1.6e-57 TIGR00231: small GTP-binding protein domain" amino acids 219 to 365 (147 residues), 81 bits, see alignment E=8.5e-27 PF02421: FeoB_N" amino acids 221 to 368 (148 residues), 42.3 bits, see alignment E=2.4e-14 PF00071: Ras" amino acids 221 to 373 (153 residues), 23 bits, see alignment E=2.1e-08 PF01926: MMR_HSR1" amino acids 221 to 334 (114 residues), 93.8 bits, see alignment E=3e-30 PF02492: cobW" amino acids 270 to 344 (75 residues), 22.1 bits, see alignment E=4.1e-08

Best Hits

Swiss-Prot: 60% identical to MNME_CYTH3: tRNA modification GTPase MnmE (mnmE) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 70% identity to psn:Pedsa_1435)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z469 at UniProt or InterPro

Protein Sequence (452 amino acids)

>CA265_RS10450 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE (Pedobacter sp. GW460-11-11-14-LB5)
MNNQDTIIALSTPSGSGAIGVIRLSGPEAISLTNAVFAGKDLEKQASHTLHFGLVKDGDH
IVDEVVAGLFVAPKSYTKENVVEISCHGSNYIIQQIINLLISKGARAAKPGEFTLRAFLN
GAFDLSQAEAVADLIASNSKASHDVAMQQMRGGFANELKGLREQLIHFASMIELELDFAE
EDVEFANREQLKNLVNKINYVLQRLISSFEMGNVIKNGVPIVIAGKPNVGKSTLLNALLN
EERAIVSDIAGTTRDTIEDELTIGGIVFRFIDTAGIRDTADIIEALGVERTLEKMKQAKL
IIYMADAAQSISEIEEQIRGLKQLAIPYLILVNKADLMADAQRKAFEALEVVFISAKEKQ
GIDELKTTLLEQVNLHHINTSETLVTNIRHVEALKQTEHALQRVLANVDNPVTSDFLAMD
IKQALHYLGEITGTVTTDDLLENIFTKFCIGK