Protein Info for CA265_RS09995 in Pedobacter sp. GW460-11-11-14-LB5

Updated annotation (from data): Nitrite reductase (NAD(P)H) (EC 1.7.1.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrite reductase (NAD(P)H)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 PF07992: Pyr_redox_2" amino acids 5 to 284 (280 residues), 192 bits, see alignment E=4.7e-60 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 800 (795 residues), 1053.9 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 148 to 224 (77 residues), 51.9 bits, see alignment E=2.7e-17 PF18267: Rubredoxin_C" amino acids 317 to 381 (65 residues), 36.8 bits, see alignment 9.7e-13 PF04324: Fer2_BFD" amino acids 421 to 469 (49 residues), 45.4 bits, see alignment 2.5e-15 PF03460: NIR_SIR_ferr" amino acids 559 to 621 (63 residues), 45 bits, see alignment 2.1e-15 PF01077: NIR_SIR" amino acids 631 to 766 (136 residues), 83.6 bits, see alignment E=3.3e-27

Best Hits

Swiss-Prot: 52% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 70% identity to cpi:Cpin_2384)

MetaCyc: 52% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z4G5 at UniProt or InterPro

Protein Sequence (837 amino acids)

>CA265_RS09995 Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (Pedobacter sp. GW460-11-11-14-LB5)
MKEPQIIVIGNGMVGYKFCEKLRSKTSSFNLIVFGEEPRRAYDRVHLSEYFNGKTADDLS
LSTENWYQEQNITLFLNNPITKIDRELQKVYTANGLDYSYDILVFATGSGAFVPNIPGIE
KEGVFVYRTIEDLDLISNYAKKAKTASVIGGGLLGLEAAKALIDLGIEKTSIIEFAPRLM
PRQIDAAGSDMLKSKLADLGLQIHLSKNTTSIEGDGRITALKFSDDTALDVDMLVISAGI
KPRDELAKACGIEVGPRGGIVVDEKMQTSDPAVFAIGECALYDGMIYGLIAPGYEMAEVL
AANLCEGDKTFTGFDMSTKLKLIGIDVASFGDNFITEPDCRTIIFENKHKGVYKRINVSN
DGQYLLGGILIGDATAYNLLLQTSNNRIVLPENPEELILGARGGSEAAGGAGITGLPDSA
LICSCEGVSKGDICTAVADGTCENIDDLKKCTKAGTGCGGCLPMVKDLMTYTLKSQGKYI
KNVICEHFNYSRQELFDLIHIHELKSYDEVLDALGKNDGCEVCKPLVSSLLASLWNEMIL
KKGNDVAQDSNDRFLANIQKGGSYSVVPRVPGGEITPDKLIVIGEVAKKYNLYTKITGGQ
RIDLFGAHLNDLPIIWEELIAAGFESGHAYGKGLRTVKSCVGSTWCRFGLHDSVSFAIRI
EERYRGIRAPHKFKSAVSGCIRECAEAQSKDFGIIATEKGWNLYVCGNGGSKPQHALLLA
TDLDSETCIQYIDRFLMFYIRTADPLTRTATWLNKMEGGIDYLRNVIINDSLGMAAQWES
EIENLIATYKCEWKEAVENPAIRKRFSHFVNAPEEKDPTIEFVEMRGQKRTAEWKTF