Protein Info for CA265_RS07955 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 14 to 535 (522 residues), 434.8 bits, E=2.8e-134
• PF13522: GATase_6
  • amino acids 28 to 154 (127 residues), 144 bits, E=6.7e-46
• PF13537: GATase_7
  • amino acids 43 to 159 (117 residues), 138.3 bits, E=3.3e-44
• PF00733: Asn_synthase
  • amino acids 239 to 599 (361 residues), 310.9 bits, E=4.3e-96
• PF06508: QueC
  • amino acids 256 to 313 (58 residues), 29.3 bits, 1.5e-10

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 67% identity to psn:Pedsa_0827)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See A0A1X9Z2Y5 at UniProt or InterPro

Protein Sequence (613 amino acids)

>CA265_RS07955 asparagine synthase (glutamine-hydrolyzing) (Pedobacter sp. GW460-11-11-14-LB5)
MCGIYGTTIPYSDGIVREKMARISFRGPDYTGVQRYDQVIFAHNRLAIIDLDARSNQPFS
YMHLHIVFNGEIYNYKDIRRELERKNYQFNTSSDTEVICAAYLEYGAGCLTRFNGMFAFV
IYDTRKNELFGARDRFGKKPFYYAHDHSSFEFASQPSQINIDRGLSVNEEAINQYLIWGY
VPEPQSIYAEVKQLPAAHYFKYDLKSHKLKITEYWTLDYGWKNKFEGSYEEAKEALALIL
ADAVKIRMNADVPLGVFLSGGIDSSLIAALATKEIDHVKTFSIKFNEKGFDESAYASQVA
KHLGTDHYTIECNYNDGIELIENFNRFYDEPFADSSAIPTMLLSKNTRKHVTVALSGDAG
DEAFLGYHRYKWIKQVNSLFYAPHGVRKALAGLINQSPSYRHKLIAMGISMEDVEKLYVK
MFGNMENSWVKQPDLAKYNPLMHILANPSKPLLERISDFDIKTYLNGDINTKVDRASMAF
SLESRSPLMDYRVMEFSRSLPTAFKYTNGNQKRILKDLLFDHVPAHMFDRPKAGFTMPFQ
HWFRNELKDYVMDNLSADNLKNIPGIHLKRTQEIINEHMTGKWNRYPQIWKLLVLSQWLI
KNKSTTHKVSLVA