Protein Info for CA265_RS07115 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 853 TIGR01051: DNA topoisomerase I" amino acids 5 to 486 (482 residues), 612.6 bits, see alignment E=6.2e-188 PF01751: Toprim" amino acids 5 to 113 (109 residues), 69.4 bits, see alignment E=3.8e-23 PF01131: Topoisom_bac" amino acids 128 to 485 (358 residues), 364.8 bits, see alignment E=1e-112 PF13368: Toprim_C_rpt" amino acids 595 to 650 (56 residues), 40.6 bits, see alignment 3.1e-14 amino acids 657 to 702 (46 residues), 58.2 bits, see alignment 1e-19

Best Hits

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 81% identity to phe:Phep_2189)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z311 at UniProt or InterPro

Protein Sequence (853 amino acids)

>CA265_RS07115 DNA topoisomerase I (Pedobacter sp. GW460-11-11-14-LB5)
MAKNLLIVESPAKAKTIEGYLGKDFLVKSSYGHIRDLVKGDMAIDIKNNFAQTYEVPADK
KNVVAELKKLAKEAEMVWLASDEDREGEAISWHLFETLGLKVEKTKRIVFHEITKPAILK
AIESPRGIDYNLVNAQQARRVLDRLVGFELSPVLWKKVKPSLSAGRVQSVAVRLIVDRER
EVLNFNAAAAYKITAQFSTGKGRELVKAELPQRFENEADAEKFLQDCVNARFDITSLETR
PAKRNPAAPFTTSTLQQEASRKLGFSVARTMQVAQRLYEAGKITYMRTDSVNLSETALNA
AAAEIKSAYGDKYHQQRIYKTKSAGAQEAHEAIRPTYFDRHSVDGDISEKRLYDLIWKRS
IASQMSEALFEKTTAQITASTRKEYLVAEGEVLKFDGFLKVYLESTDDEENEEKEGGAIL
PPLSKGQELFLKEMQATERYSRPPARYTEASLVKKLEELGIGRPSTYAPTISTVQNRGYV
VKEDRDGKQRKFTSIILADGQVKKETKSEITGAEKSKLFPTDIGEVVNDFLVEHFKGIVD
FNFTAKVEKEFDEIAQGLQEWTKMLHSFYNPFHTEVETTLETAERATGERLLGLDPATGK
NVYTKVGKFGPLVQIGELDEEEKPRYASLMRTQSVATITLDDALELFKLPFQLPDFEGKE
VMIGVGRFGPYVKWGESFISLPKNEEPLSVTYERAVEIIRQKMDDDAPIAYYEGLGVTKG
KGRFGPFIKWNDLFINIPAKGYDFDNLTQEDINTLIGKKVEKEANRYIKTWDAEKISIEN
GRWGPFIRFGKLMLKLRNNEATKQKYTPEELVDIDLEVVKKMIVEQVPNAFETKKKAPAK
KKAPAKKAVAKKK