Protein Info for CA265_RS06790 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: chondroitinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF08124: Lyase_8_N" amino acids 27 to 321 (295 residues), 255.1 bits, see alignment E=1.4e-79 PF02278: Lyase_8" amino acids 340 to 587 (248 residues), 286 bits, see alignment E=4.3e-89 PF02884: Lyase_8_C" amino acids 603 to 666 (64 residues), 68.5 bits, see alignment E=8.3e-23

Best Hits

Swiss-Prot: 65% identical to CSLA_PEDHD: Chondroitinase-AC (cslA) from Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)

KEGG orthology group: None (inferred from 65% identity to phe:Phep_0786)

MetaCyc: 65% identical to chondroitin AC lyase (Pedobacter heparinus)
Chondroitin-sulfate-ABC endolyase. [EC: 4.2.2.20, 4.2.2.5]; 4.2.2.20,4.2.2.5 [EC: 4.2.2.20, 4.2.2.5]

Predicted SEED Role

"chondroitinase AC precursor"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.2.20 or 4.2.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z2Y0 at UniProt or InterPro

Protein Sequence (701 amino acids)

>CA265_RS06790 chondroitinase (Pedobacter sp. GW460-11-11-14-LB5)
MNKLLNTGLLFLWMCLPNVIRAQQTETTKLIMDRIINDLKRPAKSVDVAAAVSLKTIQAD
GSWKDIPYQDKEITKWQPARHLVNLENLIQAYVGKESRYFEDEKVFDGIGRSFQYWYDHD
PKSSNWWHNEIATPQALGEMLIMMRYGKEHLDVNLENKLIERMKRGDIGKQTGANKTDVA
LHYFYRALLTNNQDLLRFSTDQLFEPIQLVYRKEGLQYDYSYLQHGPQLQISSYGSVFIT
GVLKLANYVRGTEYGLNGEKLELFSKYYRNSYLKAIRGSYIDFNVEGRGISRPNILDKSN
ERNRLLVAQLLDFKNTSDWNAAIARTENKETPDYKIQPYHQHFWNGDYVQHGRSTYAFNV
RMVSKRTKRSESGNKENLLGRYLSDGATNIQLRGPEYYNIMPVWEWDKIPGVTSRDYLVD
RPLTQFWGEQGSNDFAGGVSDGVYGASGYMLDYDSLQAKKSWFFFDKEIVCLGAGINSNA
PESVTTTVNQCWLNGEVLGADHEQIAGGKVAVFNDKHPSWLLHDGVGYYFPEQNNLTLST
ETQKGNWYHINNAHAKSDVSGQVFKLWINHGAKPVDAKYSYIILPGITKAGELKNFKASD
LIIVANTEKIQAVYHKALDIFQVIFYQAGTITLAGMEISTDKACALMVNHVNGNQLWSVA
DPLQKELLINMICKDVKTGKIKKLSAELPQRELAGSTVLLH