Protein Info for CA265_RS04855 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: sialate O-acetylesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 690 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00657: Lipase_GDSL" amino acids 26 to 208 (183 residues), 36.1 bits, see alignment E=1.1e-12 PF13472: Lipase_GDSL_2" amino acids 28 to 202 (175 residues), 87.2 bits, see alignment E=3e-28 PF03629: SASA" amino acids 303 to 420 (118 residues), 30.5 bits, see alignment E=4.4e-11 amino acids 481 to 579 (99 residues), 41.4 bits, see alignment E=2e-14

Best Hits

KEGG orthology group: K05970, sialate O-acetylesterase [EC: 3.1.1.53] (inferred from 60% identity to phe:Phep_3252)

Predicted SEED Role

"Sialic acid-specific 9-O-acetylesterase"

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.53

Use Curated BLAST to search for 3.1.1.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z1Y0 at UniProt or InterPro

Protein Sequence (690 amino acids)

>CA265_RS04855 sialate O-acetylesterase (Pedobacter sp. GW460-11-11-14-LB5)
MKAFFGLITGLIFLISGNIYAQKVKVACVGNSVTAGYLLPNPKQESYPAQLQQLLGDQYL
VGNFGHSGATLLKKGHNPYFKTKEFNDALNFQADIVIIHLGLNDTDPRNWPNYKGDFQAD
YQWLIDSLKKGNPKLKIYCCKLSPIFSGHPRFKSGTRDWYGQAQDKIEGVAKANQFELID
LNNALNNRPELFADNLHPDINGAGIIAKTVYAHITGNFGGFKLNTLFADHMVLQRDRPIP
VYGTANAGDWIEVQFNHITRRTITDRNGQWKVFFKPMKFGGPYLLKVKSNQHAVWLKDVM
IGDVWLCSGQSNMAFPLKSSATAQATLSRLNKNLPVRLLKYNLLAETDNIAWDEKTLKQV
NELNYFAGTWAKPDENNVGSFSAVAYYFGEKVVSNTQIPIGLIQMAVGGSTLESWMDRKT
LEHDNLLVDLLANWRKSDFIQDWVRGRANVNLKNALIPKQRHPYEPAYNYEAGISNLIGF
PIKGVIWYQGESNAHNIELYNHEFPLLVKSWREKWNGNFPFYSVQLSSIDRPSWPFFRDA
QRKLQAVIPNSGMAVSSDLGDSLNVHPTHKKEIGERLALLALKNTYHQNISAIGPVAVKA
VRKGNTIMINFSHAKRLMLGNGPVLKGFEVADEKGNHLPIRATIINNQVHLIVPAQLKIK
QVLYAWQPFTRANLINEAKLPASTFSISIN