Protein Info for CA265_RS02145 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: 1,4-alpha-glucan branching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 654 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 33 to 651 (619 residues), 833.3 bits, see alignment E=6.3e-255 PF02922: CBM_48" amino acids 49 to 132 (84 residues), 73.2 bits, see alignment E=2.6e-24 PF00128: Alpha-amylase" amino acids 203 to 270 (68 residues), 41.4 bits, see alignment E=2.2e-14 PF02806: Alpha-amylase_C" amino acids 555 to 653 (99 residues), 76.1 bits, see alignment E=3.4e-25

Best Hits

Swiss-Prot: 56% identical to GLGB_AQUAE: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Aquifex aeolicus (strain VF5)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 71% identity to phe:Phep_3308)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z0R7 at UniProt or InterPro

Protein Sequence (654 amino acids)

>CA265_RS02145 1,4-alpha-glucan branching enzyme (Pedobacter sp. GW460-11-11-14-LB5)
MPSAKKVPVKKSSQPKIDKQKSVWIHSLFTDYDIDLFLVGKHFRLYEKMGSHLITVDGVA
GTYFSVWAPNAIRVSVVGNFNDWNNASHPLSVRWDKSGIWEGFIPGIAKGEVYKYFVKGF
DESEHLKGDPYARRWEHPPQTACIVWDTDYTWKDKAWLNKRAKINALDQPISVYEIHLGS
WERDPDNPERVLNCREVAGRLVPYVKEMGFTHVELMPVMEFPFFPSWGYQITGYFGASSR
YGSPQDLMYLIDELHKAGIAVILDWVPSHFPGDRHGLYEFDGTHLYEHADMRKGFHPDWK
SYIFNYDRNEVRSFLISNALFWIDQYHADGLRVDAVASMLYFNFSREDGDAATNEYGGSE
NLGAIQFLQDLNVAVYGNFEGVQTIAEESSTYPGVTHPVYAGGLGFGMKWMMGWMNDTLK
YFKVETLGRKHHHHQLSFSMTYAFTENFMLPFSHDEVVHGKSPMLYKMPGDDWQKFANLR
ALYAYMFTHPGAKLLFMGNEFGETNEWNFTQSLDWHLLQYPPHKGMQETVKALNFLYRKE
PALYHFNFSYEGFQWLDADNANESVFTFMRKGPKAKDTLVIAINLTPVVRENYRIGVPFK
TKWTEIFNTDDIVYYGGGINNRGDIVPDLENYNGQEYSIIVDLPPLAVTVFKSK