Protein Info for CA265_RS00580 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase" amino acids 5 to 202 (198 residues), 240.5 bits, see alignment E=1.2e-75 PF00926: DHBP_synthase" amino acids 9 to 201 (193 residues), 283.3 bits, see alignment E=8.1e-89 PF00925: GTP_cyclohydro2" amino acids 212 to 376 (165 residues), 236.9 bits, see alignment E=8.7e-75 TIGR00505: GTP cyclohydrolase II" amino acids 215 to 400 (186 residues), 243.2 bits, see alignment E=1.5e-76

Best Hits

Swiss-Prot: 69% identical to RIBBA_CYTH3: Riboflavin biosynthesis protein RibBA (ribBA) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 87% identity to phe:Phep_3561)

MetaCyc: 46% identical to GTP cyclohydrolase II (Chlamydia trachomatis)
GTP cyclohydrolase II. [EC: 3.5.4.25]

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Riboflavin, FMN and FAD metabolism or Molybdenum cofactor biosynthesis (EC 3.5.4.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YZ37 at UniProt or InterPro

Protein Sequence (403 amino acids)

>CA265_RS00580 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II (Pedobacter sp. GW460-11-11-14-LB5)
MQTKLNTIEEAIADIKAGKVIIVVDDENRENEGDFLTAAENATPEIINFMATYGRGLICA
PLTEERCKELNLNLMVGKNTAAYETNFTVSVDLVGHGCTTGISASDRSKTMLALVNPKTN
PEELGRPGHIFPLIAKDGGVIRRAGHTEAAVDLARLAGMKPAGLLVEILKEDGEMARLPD
LFKIAEQHQLKIISIEDLIAYRLTIDSLIKQEVSIDLPTAWGDFKMTAYTQLDNNATHLA
ISKGEWNANEPILARVHSSCVTGDIFGSCRCDCGPQLHKALEMIEKEGKGIVVYMNQEGR
GIGLINKLRSYNLQDAGFDTVEANIKLGFKGDERDYGVGAQILRSEGVTKMRLMSNNPTK
RAGLIGYGLEVVENIPIEIASNVHNERYLTTKRDKMGHSIMKG