Protein Info for BWI76_RS27845 in Klebsiella michiganensis M5al

Annotation: multidrug transporter EmrD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 44 to 63 (20 residues), see Phobius details amino acids 75 to 92 (18 residues), see Phobius details amino acids 98 to 117 (20 residues), see Phobius details amino acids 136 to 154 (19 residues), see Phobius details amino acids 160 to 182 (23 residues), see Phobius details amino acids 203 to 226 (24 residues), see Phobius details amino acids 232 to 262 (31 residues), see Phobius details amino acids 273 to 292 (20 residues), see Phobius details amino acids 298 to 319 (22 residues), see Phobius details amino acids 330 to 353 (24 residues), see Phobius details amino acids 365 to 384 (20 residues), see Phobius details PF07690: MFS_1" amino acids 14 to 349 (336 residues), 140.5 bits, see alignment E=1e-44 PF00083: Sugar_tr" amino acids 47 to 186 (140 residues), 28.3 bits, see alignment E=1.3e-10 TIGR00880: multidrug resistance protein" amino acids 51 to 186 (136 residues), 131.7 bits, see alignment E=1.1e-42 PF06609: TRI12" amino acids 52 to 173 (122 residues), 23.2 bits, see alignment E=3.4e-09

Best Hits

Swiss-Prot: 89% identical to EMRD_ECOLI: Multidrug resistance protein D (emrD) from Escherichia coli (strain K12)

KEGG orthology group: K08154, MFS transporter, DHA1 family, 2-module integral membrane pump EmrD (inferred from 95% identity to kpu:KP1_5455)

MetaCyc: 89% identical to multidrug efflux pump EmrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-339; TRANS-RXN-44

Predicted SEED Role

"Multidrug resistance protein D"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AV89 at UniProt or InterPro

Protein Sequence (394 amino acids)

>BWI76_RS27845 multidrug transporter EmrD (Klebsiella michiganensis M5al)
MKRHKNFNLLLMLVLLVAVGQMAQTIYIPAIADMAVSLNVREGAVQSVMAAYLLTYGVSQ
LFYGPLSDRVGRRPVILVGMSIFMLATLVAITTHSLPVLIAASAMQGMGTGVGGVMARTL
PRDLYEGAQLRHANSLLNMGILVSPLLAPLIGGILDTLWNWRACFVFLLVLCAGVTFSMA
RWMPETRPTGAPRTRLITNYKTLFGTGAFNCYLLMLIGGLAGIAVFEACSGVLMGAVLGL
SSMSVSILFILPIPAAFFGAWFAGRPNKRFPTLMWQSVICCLLAGVLMWIPGLLGVMTVW
TLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGTAGALVGGLQNIGSGVLAWLSAMMPQT
GQGSLGLLMMLMGLLILLCWLPLASRFHQHQLPV