Protein Info for BWI76_RS27690 in Klebsiella michiganensis M5al

Annotation: MexE family multidrug efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 33 to 364 (332 residues), 267.2 bits, see alignment E=8.6e-84 PF13533: Biotin_lipoyl_2" amino acids 59 to 105 (47 residues), 29.7 bits, see alignment 8.4e-11 PF16576: HlyD_D23" amino acids 59 to 282 (224 residues), 47.6 bits, see alignment E=2.6e-16 PF00529: CusB_dom_1" amino acids 114 to 350 (237 residues), 41.4 bits, see alignment E=2.2e-14 PF13437: HlyD_3" amino acids 168 to 279 (112 residues), 28.6 bits, see alignment E=3.9e-10

Best Hits

Swiss-Prot: 49% identical to ACRA_ECO57: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli O157:H7

KEGG orthology group: K03585, membrane fusion protein (inferred from 90% identity to kva:Kvar_0061)

MetaCyc: 49% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Probable RND efflux membrane fusion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BBU3 at UniProt or InterPro

Protein Sequence (374 amino acids)

>BWI76_RS27690 MexE family multidrug efflux RND transporter periplasmic adaptor subunit (Klebsiella michiganensis M5al)
MKYTLAPIAAALFLLSGCDNAQNASPQQMTQEVGVVTLQGQPVPVVSSLTGRTTASLSAE
VRPQVGGIVQKRLFTEGDMVKAGQALYQIDPSSYRATFNEAAASLKQAQALVTADCQKAQ
RYAVLVKDNGVSRQDADDAIATCNQDKASVESKKAALESARINLNWTTVTAPIAGRIGIS
SVTPGALVSADQDTALATIRGLDTMYVDLTRSSVDLLRLRKQSLASNSDTLNVTLTLEDG
STYSEKGRLALTEVAVDESTGSVTLRAVFPNPQHVLLPGMFVRARIDEGIMNDAILAPQQ
GITRDAKGDATALVVDANNKVEQRSVETGETYGDKWLIVNGLKSGDRLIVEGTGKVAPGQ
QVKAAEVKNDGGNA