Protein Info for BWI76_RS27410 in Klebsiella michiganensis M5al

Annotation: DNA ligase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF01653: DNA_ligase_aden" amino acids 119 to 307 (189 residues), 67.3 bits, see alignment E=2e-22 PF03120: DNA_ligase_OB" amino acids 313 to 387 (75 residues), 66.1 bits, see alignment E=3.2e-22

Best Hits

Swiss-Prot: 66% identical to LIGB_KLEP7: DNA ligase B (ligB) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 66% identity to kpu:KP1_5350)

MetaCyc: 55% identical to DNA ligase B (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2), LigB" (EC 6.5.1.2)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.2

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BBV9 at UniProt or InterPro

Protein Sequence (558 amino acids)

>BWI76_RS27410 DNA ligase B (Klebsiella michiganensis M5al)
MQKGVWALVLWMLVGYGQAACPAWPQAKAEQEMERLGQRITEWNNAYWQQGSSTVSDEVY
DQLAARLAYWQRCFTGETPAHSALPPLRGDTRHPVAHTGVRKLANQADVARWMRGQSGLW
VQPKVDGVAITLVYRQGRLTQAISRGNGLTGEDWTARVLQIPSIPKTSDGVLANSVLQGE
LFLLREGHVQKRMGGMNARSKVAGMMMRQEAQAELDRLGIFIWAWPDGPEDMEQRLALLR
QGGFLYSTLFSHPVADAQQVERWRQRWLTSPLPFATDGVVVRRAKESAGRYWAPGQGDWV
IAWKYPPASRVMEVRGISFSVGRSGKIAVVALLEPQLLDDKRVQRVNVGSVSRWYNLDIG
VGDQLQISLAGQGIPRIDSVVWRTAQRNKPQPPAARFNALTCYFATPECAEQFLSRLVWL
SSRSVLDIDGAGEALWRTLHDARSMEHLFSWLAFTPERLQSIPGISAQRGQRLWHQFNLA
RERPFLRWIQAIGVPIPKMAFAGLKEDDWRRMQDRNEEQWRRLPGIGAERARQLVTFLHH
PDVAALAKWLSGQRVPGF