Protein Info for BWI76_RS27185 in Klebsiella michiganensis M5al

Updated annotation (from data): L-lactate/D-lactate permease
Rationale: Specific phenotype: utilization of D-Lactate, L-Lactate. (Some homologs also transport glycolate)
Original annotation: L-lactate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 551 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 40 to 58 (19 residues), see Phobius details amino acids 70 to 93 (24 residues), see Phobius details amino acids 116 to 149 (34 residues), see Phobius details amino acids 154 to 179 (26 residues), see Phobius details amino acids 199 to 217 (19 residues), see Phobius details amino acids 224 to 240 (17 residues), see Phobius details amino acids 249 to 269 (21 residues), see Phobius details amino acids 299 to 318 (20 residues), see Phobius details amino acids 368 to 387 (20 residues), see Phobius details amino acids 407 to 425 (19 residues), see Phobius details amino acids 432 to 456 (25 residues), see Phobius details amino acids 466 to 487 (22 residues), see Phobius details amino acids 499 to 515 (17 residues), see Phobius details amino acids 528 to 550 (23 residues), see Phobius details TIGR00795: transporter, lactate permease (LctP) family" amino acids 6 to 544 (539 residues), 788.6 bits, see alignment E=1.7e-241 PF02652: Lactate_perm" amino acids 16 to 542 (527 residues), 772.5 bits, see alignment E=1.2e-236

Best Hits

Swiss-Prot: 95% identical to LLDP_SALTY: L-lactate permease (lldP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00427, L-lactate permease (inferred from 97% identity to kpu:KP1_5296)

MetaCyc: 93% identical to lactate/glycolate:H+ symporter LldP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-104; TRANS-RXN-105; TRANS-RXN0-515; TRANS-RXN0-622

Predicted SEED Role

"L-lactate permease" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BB71 at UniProt or InterPro

Protein Sequence (551 amino acids)

>BWI76_RS27185 L-lactate/D-lactate permease (Klebsiella michiganensis M5al)
MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAATWTVVIALSVALLFYKM
PVDHALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLI
VGFSFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLCLIVNTAPVAFGAMGIPILVA
GQVTGLDSFEIGQMVGRQLPFLTIIVLFWIMAIMDGWRGIKETWPAVMVAGGSFAIAQYL
SSNFLGPELPDIISSLVSLVCLTLFLKRWQPVRIFRFGDMGASQVDMNLARTRYTPGQVI
RAWSPFLFLTATVTLWSVPPFKALFAPGGAMYDFVINISVPFLDKMVARMPPVVNEATPY
AAVYKFDWLSATGTAILFAALLSIVWLRMKPKDAISTFGSMLKELALPIYSIGMVLAFAF
ISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV
SDILLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCMVGVITT
LQAYVLTWMIP