Protein Info for BWI76_RS27145 in Klebsiella michiganensis M5al

Annotation: glutathione S-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13417: GST_N_3
  • amino acids 3 to 73 (71 residues), 55.8 bits, E=9.3e-19
• PF02798: GST_N
  • amino acids 3 to 71 (69 residues), 27.7 bits, E=5.4e-10
• PF13409: GST_N_2
  • amino acids 8 to 72 (65 residues), 55.6 bits, E=1.3e-18
• PF00043: GST_C
  • amino acids 99 to 191 (93 residues), 29.8 bits, E=1.2e-10

Best Hits

Swiss-Prot: 70% identical to YIBF_ECOLI: Uncharacterized GST-like protein YibF (yibF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 84% identity to kpn:KPN_03940)

Predicted SEED Role

"Glutathione S-transferase, theta (EC 2.5.1.18)" in subsystem Glutathione: Non-redox reactions (EC 2.5.1.18)

MetaCyc Pathways

• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• pentachlorophenol degradation (3/10 steps found)
• gliotoxin biosynthesis (1/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18

Sequence Analysis Tools

See A0A285BBZ9 at UniProt or InterPro

Protein Sequence (202 amino acids)

>BWI76_RS27145 glutathione S-transferase (Klebsiella michiganensis M5al)
MKLIGSYTSPFVRKISIILLEKAIPFEFVNEFPYHETNGVAQYNPLGKVPALVTDDGECW
YDSPIIAQYLEQLGIAPDMLPAAPKAALKLRQIEALADGVMEAAQMLVREKTRPAAQQSE
SELLRQREKIARGLDALEGYANDGTLRPEEVNLATISTACAIAYLNFRRVAPGWCATRPK
LVRLVDSLFQRASFARTEPPVS